LOCUS       sequence-166320-         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ER calcium sensor for lentivirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Henderson MJ, Baldwin HA, Werley CA, Boccardo S, Whitaker LR, Yan X,
            Holt GT, Schreiter ER, Looger LL, Cohen AE, Kim DS, Harvey BK
  TITLE     A Low Affinity GCaMP3 Variant (GCaMPer) for Imaging the Endoplasmic 
            Reticulum Calcium Store.
  JOURNAL   PLoS One. 2015 Oct 9;10(10):e0139273. doi: 
            10.1371/journal.pone.0139273. eCollection 2015.
  PUBMED    26451944
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-10-9"; volume: "10(10):e0139273. doi"; pages: " 
            10.1371/journal.pone.0139273. eCollection 2015"
COMMENT     Sequence Label: pMOS003-lenti-CMV-GCaMPer
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             107..124
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             128..160
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(164..172,173..187)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      194..203
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             215..271
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     484..505
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(611..632)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(872..891)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tgttttgacc tccatagaag acaccgactc tagaggatcc gccaccatgc tgctatccgt
       61 gccgttgctg ctcggcctcc tcggcctggc cgtcgccatg ggttctcatc atcatcatca
      121 tcatggtatg gctagcatga ctggtggaca gcaaatgggt cgggatctgt acgacgatga
      181 cgataaggat ctcgccacca tggtcgactc atcacgtcgt aagtggaata agacaggtca
      241 cgcagtcaga gctataggtc ggctgagctc actcgagaac gtctatatca aggccgacaa
      301 gcagaagaac ggcatcaagg cgaacttcaa gatccgccac aacatcgagg acggcggcgt
      361 gcagctcgcc taccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
      421 cgacaaccac tacctgagcg tgcagtccaa actttcgaaa gaccccaacg agaagcgcga
      481 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
      541 gtacaagggc ggtaccggag ggagcatggt gagcaagggc gaggagctgt tcaccggggt
      601 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
      661 cgagggtgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
      721 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
      781 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
      841 ctacatccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
      901 ggtgaagttc gagggcgaca ccc
//
