LOCUS       sequence-166327-         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the coding region to express the EcoRI restriction enzyme. 
            The RTX tag allows the EcoRI enzyme to be isolated via 
            calcium-precipitation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 103 to 137)
  AUTHORS   Hendrix J, Read T, Lalonde JF, Jensen PK, Heymann W, Lovelace E, 
            Zimmermann SA, Brasino M, Rokicki J, Dowell RD
  TITLE     Engineered calcium-precipitable restriction enzyme.
  JOURNAL   ACS Synth Biol. 2014 Dec 19;3(12):969-71. doi: 10.1021/sb500042m.
  PUBMED    25524101
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "ACS Synth Biol"; date: "2014-12-19- 19"; volume: "3"; issue: "12"; 
            pages: "969-71"
COMMENT     Sequence Label: pSB1A3 EcoRI-RTX with EcoRI Methylase-AmilCP
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..31
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(35..78)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    81..102
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        103..137
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     RBS             146..157
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct
       61 ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agttgacggc tagctcagtc
      121 ctaggtacag tgctagctac tagagaaaga ggagaaatac tagatggccg gcgcacgcaa
      181 cgcgaccaat aagctcctgc acaaagcgaa aaaaagcaaa agcgatgagt tttataccca
      241 gtattgcgac attgaaaacg aattacaata ctaccgcgaa catttcagcg ataaggttgt
      301 gtactgcaac tgtgatgacc cgcgtgtgag caattttttt aaatatttcg cggtgaactt
      361 tgacaacctc ggactgaaga agctgattgc aagctgttat gtagaaaaca aggagggctt
      421 tagctccagc gaagcagcaa aaaatggctt ttactatgaa taccataaag aaaacggcaa
      481 aaaactggtc ttcgatgata tttcggtgag tagcttctgt ggcgacggag attttcggag
      541 cagtgaaagc attgatctgc tcaaaaaaag tgatatcgtg gtcacgaacc cgcctttttc
      601 actctttcgt gaatacttag atcaacttat taaatacgat aaaaagttcc ttatcatcgc
      661 caatgtaaac agcattactt ataaagaagt gtttaatctg atcaaagaaa acaagatctg
      721 gctgggagta catttaggcc ggggtgtgag tggcttcatc gttccagaac attacgaact
      781 gtacggcacc gaggcccgca ttgattcaaa tggaaaccgt attatcagcc cgaataactg
      841 tctgtggctg acgaaccttg acgtcttcat tcgccacaaa gacctgccgt taacacgtaa
      901 atactttgga aacgaaagct cgtac
//
