LOCUS       sequence-166328-         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the coding region to express the EcoRI restriction enzyme. 
            The RTX tag allows the EcoRI enzyme to be isolated via 
            calcium-precipitation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Hendrix J, Read T, Lalonde JF, Jensen PK, Heymann W, Lovelace E, 
            Zimmermann SA, Brasino M, Rokicki J, Dowell RD
  TITLE     Engineered calcium-precipitable restriction enzyme.
  JOURNAL   ACS Synth Biol. 2014 Dec 19;3(12):969-71. doi: 10.1021/sb500042m.
  PUBMED    25524101
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "ACS Synth Biol"; date: "2014-12-19- 19"; volume: "3"; issue: "12"; 
            pages: "969-71"
COMMENT     Sequence Label: pSB1A3 EcoRI-RTX with EcoRI Methylase-AmilCP
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(1..58)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    59..79
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
ORIGIN
        1 tcttttcggt tttaaagaaa aagggcaggg tggtgacacc ttgccctttt ttgccggact
       61 gcagcggccg ctactagtat taaccggtat agagggtatc gttacccgcc ccgccgtaca
      121 gtgtgtcatt cccagcaccg ccataaaggg tatcgttgcc tgccccccca tacagcgtgt
      181 cgttaccggc gcccccatac aaagtgtcgt tacctgctcc gccatacagc gtatcattgc
      241 cagcacctcc gtataatgtg tcattcccgg cgccaccata cagggtatcg ttgcccgcac
      301 cgccggtata gagggtatcg ttacccgccc cgccgtacag tgtgtcattc ccagcaccgc
      361 cataaagggt atcgttgcct gcccccccat acagcgtgtc gttaccggcg cccccataca
      421 aagtgtcgtt acctgctccg ccatacagcg tatcattgcc agcacctccg tataatgtgt
      481 cattcccggc gccaccatac agggtatcgt tgcccgcacc gccggccata ccggttttgc
      541 tagtcagctg ctcgaataga tcgcgaccca gcacgcgaag tgacgtagtg ctaatatcaa
      601 acataatctc gaacatgatt ttcgaatccc attcgcgacc atccccctgg gtataaatgc
      661 tcgccgcctg gagcataata gatttatctt tatgattcac gaatttgttt atgcacaggt
      721 tagagttgat tggcatgcca tagttagccg cggtcagacg atccagacga ttcaggatgc
      781 cgctgttata ttctaggtta accacgcgac catccggacg ggtaatgctg atattttcgg
      841 tcaagaaatt tgagccctcc agaaataaca catatggaaa atggctttct gataacatga
      901 aatttgcgat ttctgagata tttttatggc tacgctcgat ggcgttgccc gcggccatca
      961 ggtcct
//
