LOCUS       sequence-16633-e        1279 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD95_70
FEATURES             Location/Qualifiers
     source          1..1279
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             248..268
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(311..339)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 tgatcttcta actaactatt ctcatttaaa ttttcagagc ttaaaaatgg ctgaaatcac
       61 tcacaacgat ggatacgcta acaacttgga aatgaaataa gcttgcatgc ctgcaggtcg
      121 actctagagg atccccggga ttggccaaag gacccaaagg tatgtttcga atgatactaa
      181 cataacatag aacattttca ggaggaccct tgcttggagg gtaccgagct cagaaaaaat
      241 gactgctcca aagaagaagc gtaaggtacc ggtagaaaaa atgagtaaag gagaagaact
      301 tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt
      361 ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat
      421 ttgcactact ggaaaactac ctgttccatg ggtaagttta aacatatata tactaactaa
      481 ccctgattat ttaaattttc agccaacact tgtcactact ttctgttatg gtgttcaatg
      541 cttctcgaga tacccagatc atatgaaacg gcatgacttt ttcaagagtg ccatgcccga
      601 aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtaa
      661 gtttaaacag ttcggtacta actaaccata catatttaaa ttttcaggtg ctgaagtcaa
      721 gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga
      781 tggaaacatt cttggacaca aattggaata caactataac tcacacaatg tatacatcat
      841 ggcagacaaa caaaagaatg gaatcaaagt tgtaagttta aacatgattt tactaactaa
      901 ctaatctgat ttaaattttc agaacttcna aattagacac aacattgaag atggaagcgt
      961 tcactagcag acnttatcac aaaatactcc aattggcgat ggccngtctt ttaccanaca
     1021 ccnttacngn tcccncatct gcccttcgaa ganccacgaa anaagacccn tggncctcnt
     1081 ganttgnacc ctgctggata ccntggcngg agacttcaat acnttctaaa tccacgaccc
     1141 ggcctnntac actggcgggc caanaagggc ccgcccnaaa gtaacggtcc nacacgnaat
     1201 tatttcnccc cccccgncnn gatnaacana ctcaaccagg cttccnggcc cccnttntna
     1261 naaccctttt ttttttctt
//
