LOCUS       sequence-166331-        8444 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Backbone for CRISPR/Cas9 screening with single cell RNA-seq. 
            Lentiviral plasmid for cloning of gRNAs and Unique Guide Index 
            (UGI), with a BFP fluorescent marker. Does NOT include Cas9..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8444)
  AUTHORS   Jaitin DA, Weiner A, Yofe I, Lara-Astiaso D, Keren-Shaul H, David E,
            Salame TM, Tanay A, van Oudenaarden A, Amit I
  TITLE     Dissecting Immune Circuits by Linking CRISPR-Pooled Screens with 
            Single-Cell RNA-Seq.
  JOURNAL   Cell. 2016 Dec 15;167(7):1883-1896.e15. doi: 
            10.1016/j.cell.2016.11.039.
  PUBMED    27984734
REFERENCE   2  (bases 1 to 8444)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.11"; journalName: "Cell"; date: "2016-12-15- 
            15"; volume: "167"; issue: "7"; pages: "1883-1896.e15"
COMMENT     Sequence Label: CRISPseq-BFP-backbone
FEATURES             Location/Qualifiers
     source          1..8444
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     80..97
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    214..235
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(250..255,256..273,274..280)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    288..304
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     293..315
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     312..328
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     312..328
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     347..367
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        349..367
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        395..621
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             622..802
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    849..974
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1467..1700
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1885..1929
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2107..2355
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2107..2127
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2278..2297
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2372..2447
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    2503..2620
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2759..3936
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2990..3927
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3884..3904
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3943..3952
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3949..3951,3952..3954,3955..4668)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(3994..4015)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    4689..5277
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4742..4762)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5160..5171)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5351..5584
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5662..5783
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5699..5718)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5753..5772
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5823..5958
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5978..5997)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5979..5997)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6007..6024)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6007..6023)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6016..6038)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6165..6620
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6252..6271)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6462..6483
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6646..6750
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6751..6819,6820..7611)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6969..6988)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7782..8370
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8271..8290
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
       61 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
      121 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
      181 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
      241 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
      301 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
      361 ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag
      421 tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt
      481 gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg
      541 ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag
      601 tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc
      661 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
      721 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
      781 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
      841 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
      901 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
      961 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     1021 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     1081 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     1141 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     1201 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     1261 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg
     1321 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     1381 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     1441 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     1501 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     1561 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     1621 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     1681 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     1741 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     1801 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     1861 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     1921 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     1981 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     2041 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
     2101 cccagagagg gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt
     2161 agagagataa ttagaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga
     2221 cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac
     2281 tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
     2341 aaaggacgaa acaccggaga cggacgtctc tgttttagag ctagaaatag caagttaaaa
     2401 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt tttaagcttg
     2461 gcgtaactag atcttgagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2521 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2581 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2641 cagagatcca ctttggcgcc ggctcgaggg ggcccgggtg caaagatgga taaagtttta
     2701 aacagagagg aatctttgca gctaatggac cttctaggtc ttgaaaggag tgggaattgg
     2761 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
     2821 aggggtcggc aattgatccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
     2881 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
     2941 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt
     3001 gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac
     3061 ttccactggc tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag
     3121 agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct
     3181 gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc
     3241 gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa
     3301 gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg
     3361 ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg
     3421 cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc
     3481 ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt
     3541 gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg
     3601 cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc
     3661 tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag
     3721 ttctcgagct tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag
     3781 tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc
     3841 tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg
     3901 gttcaaagtt tttttcttcc atttcaggtg tcgtgacgta cggccaccat ggtgagcaag
     3961 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     4021 ggccacaagt tcagcgtgag gggcgagggc gagggcgatg ccaccaacgg caagctgacc
     4081 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     4141 ctgagccacg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc
     4201 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     4261 ggcacctaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     4321 gagctgaagg gcgtcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     4381 aacttcaaca gccacaacat ctatatcatg gccgtcaagc agaagaacgg catcaaggtg
     4441 aacttcaaga tccgccacaa cgtggaggac ggcagcgtgc agctcgccga ccactaccag
     4501 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acagccacta cctgagcacc
     4561 cagtccgtgc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     4621 cgcaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaac gcgttaagtc
     4681 gagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     4741 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     4801 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     4861 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     4921 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     4981 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     5041 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     5101 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     5161 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     5221 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcgtc
     5281 gacggatccg gtgatgggac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     5341 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     5401 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     5461 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     5521 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     5581 agcagtacgt atagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     5641 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     5701 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     5761 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     5821 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     5881 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     5941 gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc
     6001 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     6061 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     6121 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     6181 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     6241 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     6301 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     6361 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     6421 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     6481 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     6541 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     6601 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     6661 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     6721 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     6781 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     6841 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     6901 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     6961 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     7021 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     7081 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     7141 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     7201 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     7261 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     7321 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     7381 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     7441 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     7501 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     7561 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     7621 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     7681 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     7741 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     7801 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     7861 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     7921 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     7981 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     8041 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     8101 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     8161 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     8221 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     8281 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     8341 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     8401 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgtt
//
