LOCUS       sequence-16634-e        1314 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD95_73
FEATURES             Location/Qualifiers
     source          1..1314
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             243..263
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(306..334)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ctgatcttct aactaactat tctcatttaa attttcagag cttaaaaatg gctgaaatca
       61 ctcacaacga tggatacgct aacaacttgg aaatgaaata agcttgcatg cctgcaggtc
      121 gactagagga tccccgggat tggccaaagg acccaaaggt atgtttcgaa tgatactaac
      181 ataacataga acattttcag gaggaccctt ggagggtacc gagctcagaa aaaatgactg
      241 ctccaaagaa gaagcgtaag gtaccggtag aaaaaatgag taaaggagaa gaacttttca
      301 ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg
      361 tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca
      421 ctactggaaa actacctgtt ccatgggtaa gtttaaacat atatatacta actaaccctg
      481 attatttaaa ttttcagcca acacttgtca ctactttctg ttatggtgtt caatgcttct
      541 cgagataccc agatcatatg aaacggcatg actttttcaa gagtgccatg cccgaaggtt
      601 atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca cgtaagttta
      661 aacagttcgg tactaactaa ccatacatat ttaaattttc aggtgctgaa gtcaagtttg
      721 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa
      781 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag
      841 acaaacaaaa gaatggaatc aaagttgtaa gtttaaacat gattttacta actaactaat
      901 ctgatttaaa ttttcagaac ttcnaaatta gacacaacat tgaagatgga agcgtncact
      961 agcagacctt atcaccaaat actccnattg gcgatggncc tgtcctttnc canacancnt
     1021 tacctgtccn ccnatctgcc cnttncnaag atcccacgaa agaagaccnn tggncctctg
     1081 antggaacac tgcgggatac ctggcaggag acttacaata gcntctaaat ncacgacccg
     1141 ncctncntan actgnggggc aaaaaagggc nnnnccnaaa gtnacgnntc cnaccacgna
     1201 attanttcnc nccgccngcc ngattaanca ttcttaancc cnnnnttccg gncccccntt
     1261 nttaaaannn nntttttttt ttttttcccc naggngggac naaanttcca aaaa
//
