LOCUS       sequence-166342-        3918 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing SPOP, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_SPOP_p.F102C
FEATURES             Location/Qualifiers
     source          1..3918
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      37..625
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     526..545
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     779..796
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(955..982)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1074..1160)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1209..1231
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1223..1240
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1224..1240
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1256..1355
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2484..2583)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2600..2619)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2601..2619)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2624..2640)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2624..2640)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3071..3862
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
       61 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
      121 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
      181 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
      241 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
      301 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
      361 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
      421 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
      481 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
      541 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
      601 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
      661 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
      721 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
      781 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
      841 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
      901 gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg
      961 ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct
     1021 gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc
     1081 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac
     1141 tgagcctttc gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg
     1201 gatgttttcc cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccccaaat
     1261 aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgatg agcaatgctt
     1321 ttttataatg acaactttgt acaaaaaagt tggcaccatg tcaagggttc caagtcctcc
     1381 acctccggca gaaatgtcga gtggccccgt agctgagagt tggtgctaca cacagatcaa
     1441 ggtagtgaaa ttctcctaca tgtggaccat caataacttt agcttttgcc gggaggaaat
     1501 gggtgaagtc attaaaagtt ctacattttc atcaggagca aatgataaac tgaaatggtg
     1561 tttgcgagta aaccccaaag ggttagatga agaaagcaaa gattacctgt cactttacct
     1621 gttactggtc agctgtccaa agagtgaagt tcgggcaaaa tgcaaattct ccatcctgaa
     1681 tgccaaggga gaagaaacca aagctatgga gagtcaacgg gcatataggt ttgtgcaagg
     1741 caaagactgg ggattcaaga aattcatccg tagagatttt cttttggatg aggccaacgg
     1801 gcttctccct gatgacaagc ttaccctctt ctgcgaggtg agtgttgtgc aagattctgt
     1861 caacatttct ggccagaata ccatgaacat ggtaaaggtt cctgagtgcc ggctggcaga
     1921 tgagttagga ggactgtggg agaattcccg gttcacagac tgctgcttgt gtgttgccgg
     1981 ccaggaattc caggctcaca aggctatctt agcagctcgt tctccggttt ttagtgccat
     2041 gtttgaacat gaaatggagg agagcaaaaa gaatcgagtt gaaatcaatg atgtggagcc
     2101 tgaagttttt aaggaaatga tgtgcttcat ttacacgggg aaggctccaa acctcgacaa
     2161 aatggctgat gatttgctgg cagctgctga caagtatgcc ctggagcgct taaaggtcat
     2221 gtgtgaggat gccctctgca gtaacctgtc cgtggagaac gctgcagaaa ttctcatcct
     2281 ggccgacctc cacagtgcag atcagttgaa aactcaggca gtggatttca tcaactatca
     2341 tgcttcggat gtcttggaga cctctgggtg gaagtcaatg gtggtgtcac atccccactt
     2401 ggtggctgag gcataccgct ctctggcttc agcacagtgc ccttttctgg gacccccacg
     2461 caaacgcctg aagcaatcct aattgccaac tttcttgtac aaagttggca ttataagaaa
     2521 gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat
     2581 ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg
     2641 gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
     2701 atcatgcctc ctctagacca gccaggacag aaatgcctcg acttcgctgc tacccaaggt
     2761 tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagtgga cataagcctg
     2821 ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt
     2881 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
     2941 ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg
     3001 atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa
     3061 gttaaacatt atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt
     3121 tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc
     3181 agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag
     3241 gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc
     3301 tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat
     3361 tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat
     3421 tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa
     3481 agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt
     3541 tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc
     3601 cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc
     3661 agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc
     3721 ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga
     3781 tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat
     3841 caccaaggta gtcggcaaat aaccctcgag ccacccatga ccaaaatccc ttaacgtgag
     3901 ttacgcgtcg ttccactg
//
