LOCUS       sequence-166356-        3918 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing SPOP, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_SPOP_p.K134N
FEATURES             Location/Qualifiers
     source          1..3918
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      35..623
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     524..543
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     777..794
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(953..980)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1072..1158)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1207..1229
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1221..1238
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1222..1238
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1254..1353
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2482..2581)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2598..2617)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2599..2617)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2622..2638)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2622..2638)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3069..3860
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
       61 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
      121 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
      181 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
      241 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
      301 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
      361 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
      421 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
      481 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
      541 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
      601 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
      661 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
      721 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
      781 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
      841 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
      901 gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc
      961 atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc
     1021 ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct
     1081 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg
     1141 agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg ctagcatgga
     1201 tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg ccccaaataa
     1261 tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgatgag caatgctttt
     1321 ttataatgcc aactttgtac aaaaaagttg gcaccatgtc aagggttcca agtcctccac
     1381 ctccggcaga aatgtcgagt ggccccgtag ctgagagttg gtgctacaca cagatcaagg
     1441 tagtgaaatt ctcctacatg tggaccatca ataactttag cttttgccgg gaggaaatgg
     1501 gtgaagtcat taaaagttct acattttcat caggagcaaa tgataaactg aaatggtgtt
     1561 tgcgagtaaa ccccaaaggg ttagatgaag aaagcaaaga ttacctgtca ctttacctgt
     1621 tactggtcag ctgtccaaag agtgaagttc gggcaaaatt caaattctcc atcctgaatg
     1681 ccaagggaga agaaaccaaa gctatggaga gtcaacgggc atataggttt gtgcaaggca
     1741 aagactgggg attcaacaaa ttcatccgta gagattttct tttggatgag gccaacgggc
     1801 ttctccctga tgacaagctt accctcttct gcgaggtgag tgttgtgcaa gattctgtca
     1861 acatttctgg ccagaatacc atgaacatgg taaaggttcc tgagtgccgg ctggcagatg
     1921 agttaggagg actgtgggag aattcccggt tcacagactg ctgcttgtgt gttgccggcc
     1981 aggaattcca ggctcacaag gctatcttag cagctcgttc tccggttttt agtgccatgt
     2041 ttgaacatga aatggaggag agcaaaaaga atcgagttga aatcaatgat gtggagcctg
     2101 aagtttttaa ggaaatgatg tgcttcattt acacggggaa ggctccaaac ctcgacaaaa
     2161 tggctgatga tttgctggca gctgctgaca agtatgccct ggagcgctta aaggtcatgt
     2221 gtgaggatgc cctctgcagt aacctgtccg tggagaacgc tgcagaaatt ctcatcctgg
     2281 ccgacctcca cagtgcagat cagttgaaaa ctcaggcagt ggatttcatc aactatcatg
     2341 cttcggatgt cttggagacc tctgggtgga agtcaatggt ggtgtcacat ccccacttgg
     2401 tggctgaggc ataccgctct ctggcttcag cacagtgccc ttttctggga cccccacgca
     2461 aacgcctgaa gcaatcctaa ttgccaactt tcttgtacaa agttgtcatt ataagaaagc
     2521 attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt
     2581 gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc
     2641 agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat
     2701 catgcctcct ctagaccagc caggacagaa atgcctcgac ttcgctgcta cccaaggttg
     2761 ccgggtgacg cacaccgtgg aaacggatga aggcacgaac ccagtggaca taagcctgtt
     2821 cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga
     2881 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt
     2941 ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
     3001 gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt
     3061 taaacattat gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg
     3121 gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag
     3181 tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc
     3241 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg
     3301 gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc
     3361 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc
     3421 ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag
     3481 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc
     3541 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg
     3601 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag
     3661 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg
     3721 cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc
     3781 gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca
     3841 ccaaggtagt cggcaaataa ccctcgagcc acccatgacc aaaatccctt aacgtgagtt
     3901 acgcgtcgtt ccactgag
//
