LOCUS       sequence-166369-        3858 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing HAPLN1, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_HAPLN1_p.P233H
FEATURES             Location/Qualifiers
     source          1..3858
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     173..192
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     426..443
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(602..629)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(721..807)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     856..878
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     870..887
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     871..887
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    903..1002
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2071..2170)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2187..2206)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2188..2206)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2211..2227)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2211..2227)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2658..3449
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(3542..3858,1..272)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
       61 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
      121 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
      181 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
      241 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
      301 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
      361 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
      421 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
      481 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
      541 gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt gtagaaacgc
      601 aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg
      661 gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg
      721 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc
      781 ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcgttaacgc
      841 tagcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc
      901 cccaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc
      961 aatgcttttt tataatgcca actttgtaca aaaaagttgg caccatgaag agtctacttc
     1021 ttctggtgct gatttcaatc tgctgggctg atcatctttc agacaactat actctggatc
     1081 atgacagagc tattcacatc caagcagaaa atggccccca tctacttgtg gaagcagagc
     1141 aagccaaggt gttttcacac agaggtggca atgttacact gccatgtaaa ttttatcgag
     1201 accctacagc atttggctca ggaatccata aaatccgaat taagtggacc aagctaactt
     1261 cggattacct caaggaagtg gatgtttttg tttccatggg ataccacaaa aaaacctatg
     1321 gaggctacca gggtagagtg tttctgaagg gaggcagtga tagtgatgct tctctggtca
     1381 tcacagacct cactctggaa gattatggga gatataagtg tgaggtgatt gaaggattag
     1441 aagatgatac tgttgtggta gcactggact tacaaggtgt ggtattccct tactttccac
     1501 gactggggcg ctacaatctc aattttcacg aggcgcagca ggcgtgtctg gaccaggatg
     1561 ctgtgatcgc ctccttcgac cagctgtacg acgcctggcg gggcgggctg gactggtgca
     1621 atgccggctg gctcagtgat ggctctgtgc aatatcccat cacaaagccc agagagccct
     1681 gtgggggcca gaacacagtg cacggagtca ggaactacgg attttgggat aaagataaaa
     1741 gcagatatga tgttttctgt tttacatcca atttcaatgg ccgtttttac tatctgatcc
     1801 accccaccaa actgacctat gatgaagcgg tgcaagcttg tctcaatgat ggtgctcaga
     1861 ttgcaaaagt gggccagata tttgctgcct ggaaaattct cggatatgac cgctgtgatg
     1921 cgggctggtt ggcggatggc agcgtccgct accccatctc taggccaaga aggcgctgca
     1981 gtcctactga ggctgcagtg cgcttcgtgg gtttcccaga taaaaagcat aagctgtatg
     2041 gtgtctactg cttcagagca tacaactgat tgccaacttt cttgtacaaa gttggcatta
     2101 taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa
     2161 tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg
     2221 tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa
     2281 atatatcatc atgcctcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctac
     2341 ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat
     2401 aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca
     2461 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
     2521 actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
     2581 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
     2641 aaacaaagtt aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag
     2701 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
     2761 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga
     2821 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
     2881 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
     2941 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
     3001 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
     3061 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
     3121 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
     3181 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
     3241 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
     3301 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag
     3361 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag
     3421 gcgagatcac caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta
     3481 acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     3541 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     3601 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     3661 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     3721 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     3781 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     3841 aggcgcagcg gtcgggct
//
