LOCUS       sequence-166373-         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C-Flag/HA tagged HPV 16 E7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Grace M, Munger K
  TITLE     Proteomic analysis of the gamma human papillomavirus type 197 E6 and
            E7 associated cellular proteins.
  JOURNAL   Virology. 2016 Oct 20;500:71-81. doi: 10.1016/j.virol.2016.10.010.
  PUBMED    27771561
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virology.";
            date: "2016-10-20"; volume: "500:71-81. doi"; pages: " 
            10.1016/j.virol.2016.10.010"
COMMENT     Sequence Label: CMV-C 16 E7
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             473..496
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             509..535
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(634..653)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    699..754
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(753..772)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 gatatacact gtttgagatg aggataaaat actctgagtc caaaccgggc ccctctgcta
       61 accatgttca tgccttcttc tctttcctac agctcctggg caacgtgctg gttgttgtgc
      121 tgtctcatca ttttggcaaa gaattcctcg acggatccga ttcgagacca tgcatggaga
      181 tacacctaca ttgcatgaat atatgttaga tttgcaacca gagacaactg atctctactg
      241 ttatgagcaa ttaaatgaca gctcagagga ggaggatgaa atagatggtc cagctggaca
      301 agcagaaccg gacagagccc attacaatat tgtaaccttt tgttgcaagt gtgactctac
      361 gcttcggttg tgcgtacaaa gcacacacgt agacattcgt actttggaag acctgttaat
      421 gggcacacta ggaattgtgt gccccatctg ttctcagaaa ccagcggcca tcgactacaa
      481 ggatgacgat gacaagctcg atggaggata cccatacgat gttccagatt acgcttagag
      541 atccgtcgag gaattcactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt
      601 ggccaatgcc ctggctcaca aataccactg agatcttttt ccctctgcca aaaattatgg
      661 ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat
      721 tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa
      781 tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct
      841 ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc
      901 tgtccattcc ttattccata gaaaagcctt gacttgaggt agatttt
//
