LOCUS       sequence-16639-e        1291 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD95_85
FEATURES             Location/Qualifiers
     source          1..1291
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(247..275)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gngctgcggt cgactctaga ggatccccgg gattggccaa aggacccaaa ggtatgtttc
       61 gaatgatact aacataacat agaacatttt caggaggacc cttggctagc aaaaatgcat
      121 aaggttttgc tggcactgtt ctttatcttt ctggcaccag catccgcact ggcagtctcc
      181 gaaccggtcc tagcgtcgac ggtaccggta gaaaaaatga gtaaaggaga agaacttttc
      241 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
      301 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc
      361 actactggaa aactacctgt tccatgggta agtttaaaca tatatatact aactaaccct
      421 gattatttaa attttcagcc aacacttgtc actactttca cttatggtgt tcaatgcttc
      481 tcgagatacc cagatcatat gaaacggcat gactttttca agagtgccat gcccgaaggt
      541 tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac acgtaagttt
      601 aaacagttcg gtactaacta accatacata tttaaatttt caggtgctga agtcaagttt
      661 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga
      721 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca
      781 gacaaacaaa agaatggaat caaagttgta agtttaaaca tgattttact aactaactaa
      841 tctgatttaa attttcagaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa
      901 ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac
      961 aaccattacc tgtccacaca nnctgccctt tcgaaagatc ccaacgaaag aaagacccat
     1021 ggncctnctg agttgnaaca gctgctggga atacncntgg ctggatgaac tatacaaata
     1081 gcatccgtaa aatncnactg agcgccggtc nctaccatna ccaacttgnc nggggccnaa
     1141 aanaaanggg gccnccgtcn tccaaanann ttnaaccnga gttcccccac naacgggnaa
     1201 aatttaantt tncngaattc cccgcccggg nccntgaaat tnnaanncna annacccttn
     1261 aaaatcccaa angggcnttn ccccgggggc c
//
