LOCUS       sequence-166392-        6954 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HPV 197 E6 in CMV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6954)
  AUTHORS   Grace M, Munger K
  TITLE     Proteomic analysis of the gamma human papillomavirus type 197 E6 and
            E7 associated cellular proteins.
  JOURNAL   Virology. 2016 Oct 20;500:71-81. doi: 10.1016/j.virol.2016.10.010.
  PUBMED    27771561
REFERENCE   2  (bases 1 to 6954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virology.";
            date: "2016-10-20"; volume: "500:71-81. doi"; pages: " 
            10.1016/j.virol.2016.10.010"
COMMENT     Sequence Label: CMV 197 E6
FEATURES             Location/Qualifiers
     source          1..6954
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          696..1268
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(1874..1893)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1939..1994
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1993..2012)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2351..2367)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2351..2367)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2364..2386)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2375..2391
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2399..2405,2406..2423,2424..2429))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2444..2465
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2582..2599)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2753..3341)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2833..2852)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3512..4303,4304..4372))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4135..4154
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4373..4478)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4546..4564
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4602..4624)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4724..4743
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4938..4960
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4952..4969
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4953..4969
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5380..5525
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             5582..6376
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5636..5655)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6246..6265
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6570..6589)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6614..6661
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg gtcgaccgat cctgagaact tcagggtgag tttggggacc cttgattgtt
      721 ctttcttttt cgctattgta aaattcatgt tatatggagg gggcaaagtt ttcagggtgt
      781 tgtttagaat gggaagatgt cccttgtatc accatggacc ctcatgataa ttttgtttct
      841 ttcactttct actctgttga caaccattgt ctcctcttat tttcttttca ttttctgtaa
      901 ctttttcgtt aaactttagc ttgcatttgt aacgaatttt taaattcact tttgtttatt
      961 tgtcagattg taagtacttt ctctaatcac ttttttttca aggcactcag ggtatattat
     1021 attgtacttc agcacagttt tagagaacaa ttgttataat taaatgataa ggtagaatat
     1081 ttctgcatat aaattctggc tggcgtggaa atattcttat tggtagaaac aactacaccc
     1141 tggtcatcat cctgcctttc tctttatggt tacaatgata tacactgttt gagatgagga
     1201 taaaatactc tgagtccaaa ccgggcccct ctgctaacca tgttcatgcc ttcttctctt
     1261 tcctacagct cctgggcaac gtgctggttg ttgtgctgtc tcatcatttt ggcaaagaat
     1321 tcctcgacgg atccatggag cccctgctga agcctctgag tattgatgag tattgtaact
     1381 atttccacat tgattccctg tatctgaccc tgccctgtat tttctgccac aacatcattg
     1441 acagcctgca gctgaccatc tttcagcgga gacagctgtc cctggtgtgg aggggggata
     1501 attgtttcgc cgcttgcctg tcttgtatcg caaacgtggc caagctggag cgcaacaagt
     1561 actttcagtg cctggtcaag ggcgactata ttgaacattg tacccagaca cccctgcaga
     1621 acctgctggt ccgatgcctg ttctgtatgg ctctgctggg cggggacgag aaaatcgatg
     1681 tgattgccag cggagaaaac ttctacctgg tgcggggcgc ttggaagagt gtctgtagaa
     1741 actgtagcca gaacgcttgg ggagaaagta ataactaagg atccgtcgag gaattcactc
     1801 ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca
     1861 aataccactg agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg
     1921 agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt
     1981 ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg
     2041 agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt
     2101 ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata
     2161 gaaaagcctt gacttgaggt agattttttt tatattttgt tttgtgttat ttttttcttt
     2221 aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact
     2281 actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacggat cggccgcaat
     2341 tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     2401 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     2461 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     2521 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     2581 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     2641 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     2701 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     2761 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     2821 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     2881 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2941 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     3001 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     3061 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     3121 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     3181 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     3241 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     3301 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     3361 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     3421 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     3481 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     3541 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     3601 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     3661 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     3721 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     3781 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     3841 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     3901 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     3961 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     4021 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     4081 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     4141 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     4201 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     4261 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     4321 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     4381 tttttctaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     4441 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     4501 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     4561 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     4621 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     4681 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     4741 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     4801 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     4861 gcctcttcgc tattacgcca gctggcggaa agggggatgt gctgcaaggc gattaagttg
     4921 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagcttca
     4981 cgctgccgca agcactcagg gcgcaagggc tgctaaagga agcggaacac gtagaaagcc
     5041 agtccgcaga aacggtgctg accccggatg aatgtcagct actgggctat ctggacaagg
     5101 gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg ggcttacatg gcgatagcta
     5161 gactgggcgg ttttatggac agcaagcgaa ccggaattgc cagctggggc gccctctggt
     5221 aaggttggga agccctgcaa agtaaactgg atggctttct tgccgccaag gatctgatgg
     5281 cgcaggggat caagatcctg cttcatcccc gtggcccgtt gctcgcgttt gctggcggtg
     5341 tccccggaag aaatatattt gcatgtcttt agttctatga tgacacaaac cccgcccagc
     5401 gtcttgtcat tggcgaattc gaacacgcag atgcagtcgg ggcggcgcgg tcccaggtcc
     5461 acttcgcata ttaaggtgac gcgtgtggcc tcgaacaccg agcgaccctg cagcgacccg
     5521 cttaacagcg tcaacagcgt gccgcagatc tgatcaagag acaggatgag gatcgtttcg
     5581 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     5641 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     5701 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     5761 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     5821 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     5881 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     5941 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     6001 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     6061 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     6121 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     6181 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     6241 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     6301 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     6361 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     6421 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     6481 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     6541 gcccaccccg ggagatgggg gaggctaact gaaacacgga aggagacaat accggaagga
     6601 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt
     6661 cataaacgcg gggttcggtc ccagggctgg cactctgtcg atacccccac cgagacccca
     6721 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
     6781 aggcccaggg ctcgcagcca acgtcggggc ggcaagccct gccatagcca cgggccccgt
     6841 gggttaggga cggcggatcg cggccctcta gagagcttgg cccattgcat acgttgtatc
     6901 catatcataa tatgtacatt tatattggct catgtccaac attaccgcca tgtt
//
