LOCUS       sequence-16641-e        1334 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD95_93
FEATURES             Location/Qualifiers
     source          1..1334
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             360..380
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(423..451)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gngnctgcgg tcgactctag aggatcccca attcttgaga cttccactat cttctctggc
       61 accctccact acgcgtaggc gtctctcgct tcgtgtattc ccgggaagcc gggttcccgt
      121 ctctcccgcc gctgccgctg ccgcacacag ctttacacct cgtagaatcc ccaaagaggg
      181 gcgtggcttg cgggtgccaa catcctcctg ccgaggaaga agcaggcact catcactcgc
      241 atcatcaacc tcgggattgg ccaaaggacc caaaggtatg tttcgaatga tactaacata
      301 acatagaaca ttttcaggag gacccttgga gggtaccgag ctcagaaaaa atgactgctc
      361 caaagaagaa gcgtaaggta ccggtagaaa aaatgagtaa aggagaagaa cttttcactg
      421 gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca
      481 gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta
      541 ctggaaaact acctgttcca tgggtaagtt taaacatata tatactaact aaccctgatt
      601 atttaaattt tcagccaaca cttgtcacta ctttctgtta tggtgttcaa tgcttctcga
      661 gatacccaga tcatatgaaa cggcatgact ttttcaagag tgccatgccc gaaggttatg
      721 tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt aagtttaaac
      781 agttcggtac taactaacca tacatattta aattttcagg tgctgaagtc aagtttgaag
      841 gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca
      901 ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca
      961 aacaaaagaa tggaatcaag ttgtaagttt aacatgattt tactaactaa ctaatctgat
     1021 taaattttca aactncaaat tagacncaca tngaaaanga agcgtncact agcaaccntt
     1081 tcacaaaata ctccattggc ganggcctgt ctttacaaaa acattactgt ccccantctg
     1141 ccnttcaaaa tnccacaaaa anaaccnngg ncctctgatt gaaaccngcg ggataacnng
     1201 ggnggagaaa taaaagccgg ggggnaaaca aaaancccca agggccnnat nnccngtttn
     1261 cccgtggggn aaaaaccccn tttaancngg gggnaacngg ggnccaanan ctggncaccc
     1321 cccccggggn aaaa
//
