LOCUS       sequence-166413-         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C-Flag/HA tagged HPV 197 E7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Grace M, Munger K
  TITLE     Proteomic analysis of the gamma human papillomavirus type 197 E6 and
            E7 associated cellular proteins.
  JOURNAL   Virology. 2016 Oct 20;500:71-81. doi: 10.1016/j.virol.2016.10.010.
  PUBMED    27771561
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virology.";
            date: "2016-10-20"; volume: "500:71-81. doi"; pages: " 
            10.1016/j.virol.2016.10.010"
COMMENT     Sequence Label: CMV-C 197 E7
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             455..478
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             491..517
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(616..635)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    681..736
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(735..754)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 atatacactg tttgagatga ggataaaata ctctgagtcc aaaccgggcc cctctgctaa
       61 ccatgttcat gccttcttct ctttcctaca gctcctgggc aacgtgctgg ttgttgtgct
      121 gtctcatcat tttggcaaag aattcctcga cggatccatg cacggggaaa gagcaactat
      181 tagagatgtg gtcctggaac tgacaaaggt cgcctgtaac tcagaagtcc tgtttgaaga
      241 gagcctgagc tccgactccg aggaagagca cgaaatcttc aagattgaga cccgatgcga
      301 taactgtcga agaggcatca gggtgtgcgt ccgcgcatct aatcagggga ttagtagact
      361 gcagtggctg ctggaacatg acattaggtt tctgtgcccc gcttgtagcc gcaatctgtt
      421 tcagcatgga aggtttcagt caggatccga tatcgactac aaggatgacg atgacaagct
      481 cgatggagga tacccatacg atgttccaga ttacgcttag agatccgtcg aggaattcac
      541 tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca
      601 caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct
      661 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
      721 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa
      781 tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg
      841 ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca
      901 tagaaaagcc ttgacttgag gttagatttt t
//
