LOCUS       sequence-16642-e        1214 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD96_02
FEATURES             Location/Qualifiers
     source          1..1214
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             356..376
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(419..447)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ctgcggtcga ctctagagga tccccaattc ttgagacttc cactatcttc tctggcaccc
       61 tccactacgc gtaggcgtct ctcgcttcgt gtattcccgg gaagccgggt tcccgtctct
      121 cccgccgctg ccgctgccgc acacagcttt acacctcgta gaatccccaa agaggggcgt
      181 ggcttgcggg tgccaacatc ctcctgccga ggaagaagca ggcactcatc actcgcatca
      241 tcaacctcgg gattggccaa aggacccaaa ggtatgtttc gaatgatact aacataacat
      301 agaacatttt caggaggacc cttggagggt accgagctca gaaaaaatga ctgctccaaa
      361 gaagaagcgt aaggtaccgg tagaaaaaat gagtaaagga gaagaacttt tcactggagt
      421 tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg
      481 agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg
      541 aaaactacct gttccatggg taagtttaaa catatatata ctaactaacc ctgattattt
      601 aaattttcag ccaacacttg tcactacttt ctgttatggt gttcaatgct tctcgagata
      661 cccagatcat atgaaacggc atgacttttt caagagtgcc atgcccgaag gttatgtaca
      721 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtaagt ttaaacagtt
      781 cggtactaac taaccataca tatttaaatt ttcaggtgct gaagtcaagt ttgaaggtga
      841 tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct
      901 tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg cagacaacca
      961 aagaatggaa tcnaagttgt aagtttaaac atgattttac taactaacta atctgattta
     1021 antttcagaa ctncaaatta gaccaacatn gaananggag cgttcactag caaacnttnt
     1081 cacaaatact ccattgggga tggcctgtcn tttacanaaa acnttactgn ccnccaatcg
     1141 gccnttnnaa natcccacga aaaannancc ccnggccctt ttgantngna nccggnggga
     1201 taaccnnggg ngga
//
