LOCUS       sequence-166420-        1242 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses DNA-binding (ETS) domain of murine PU.1, residues 167-272.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Wang S, Linde MH, Munde M, Carvalho VD, Wilson WD, Poon GM
  TITLE     Mechanistic heterogeneity in site recognition by the structurally 
            homologous DNA-binding domains of the ETS family transcription 
            factors Ets-1 and PU.1.
  JOURNAL   J Biol Chem. 2014 Aug 1;289(31):21605-16. doi: 
            10.1074/jbc.M114.575340. Epub 2014 Jun 21.
  PUBMED    24952944
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.575340"; journalName: "J Biol Chem"; date: 
            "2014-08-1- 1"; volume: "289"; issue: "31"; pages: "21605-16"
COMMENT     Sequence Label: pET28b-PU.1-ETS
FEATURES             Location/Qualifiers
     source          1..1242
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             371..388
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             395..412
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             435..452
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(505..523)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      519..566
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      603..1058
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(690..709)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     900..921
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cnnnntccnt ctagantant ttnnttnaac tttaagaagg agatatacca tgggcagcaa
       61 gaagaagatt cgtctgtacc aattcctgct cgatttgctg cggtcaggag atatgaaaga
      121 ctcgatctgg tgggtcgata aagacaaagg tacctttcaa ttcagttcta aacataaaga
      181 ggcattagcg cacaggtggg gaattcagaa gggcaatcgc aagaagatga cataccagaa
      241 gatggcgcgt gctctgcgca actatggcaa gactggtgaa gttaagaaag tgaagaagaa
      301 attgacgtat cagtttagcg gcgaagtatt agggcgtggt gggctggccg aaagacgcct
      361 tccgccacat ctagtgcctc gaggttccag atctcatcac catcaccatc actaagcttg
      421 cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa
      481 aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct
      541 ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga ttggcgaatg
      601 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
      661 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      721 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
      781 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
      841 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
      901 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
      961 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     1021 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt ttcggggaaa
     1081 tgtgcgcgga acccntattt ggttattttt ctaantacat tcaaatatgt atccnnntca
     1141 tgaantantt ncttagaaaa anctcatcng agcattcaaa tggaaactgc catttnntnc
     1201 nttatccggn attatccann nccnnnnttt tttgaaaaaa ca
//
