LOCUS       sequence-16643-e        1338 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD96_04
FEATURES             Location/Qualifiers
     source          1..1338
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             252..272
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(315..343)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gnntnactga tcttactaac taactattct catttaaatt ttcagagctt aaaaatggct
       61 gaaatcactc acaacgatgg atacgctaac aacttggaaa tgaaataagc ttgcatgcct
      121 gcaggtcgac tctagaggat ccccgggatt ggccaaagga cccaaaggta tgtttcgaat
      181 gatactaaca taacatagaa cattttcagg aggacccttg gagggtaccg agctcagaaa
      241 aaatgactgc tccaaagaag aagcgtaagg taccggtaga aaaaatgagt aaaggagaag
      301 aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca
      361 aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat
      421 ttatttgcac tactggaaaa ctacctgttc catgggtaag tttaaacata tatatactaa
      481 ctaaccctga ttatttaaat tttcagccaa cacttgtcac tactttctgt tatggtgttc
      541 aatgcttctc gagataccca gatcatatga aacggcatga ctttttcaag agtgccatgc
      601 ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac
      661 gtaagtttaa acagttcggt actaactaac catacatatt taaattttca ggtgctgaag
      721 tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag
      781 aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca
      841 tcatggcaga caaacaaaag aatggaatca aagttgtaag tttaaacatg attttactaa
      901 ctaactaatc tgatttaaat tttcagaact tcaaattaga cacaacattg aagatggaag
      961 cgttcactag cagacnttat cnacaaatac tccattggcg atggcctgtc ctttacagac
     1021 aacnttacct gtcccncatc tgcccttcga agatccacga aanannacnc tggncctctg
     1081 anttgnaccc tgcnggatac caggctggag actaacaann ccggggggan acaaaccccc
     1141 ncaatgaccn gaatgccccg ttcnccnntt ggnaacancc cctttannnc gggggnaacg
     1201 gggtncnata nnctggnaac ccctnccngg gnaaaaaagc cccccccncc cccggggaat
     1261 aaaaccnaaa ntttggantt nttngggggg ctccttnccc cccngcnggg ggttttnaag
     1321 ncccccccgg aaaacccc
//
