LOCUS       sequence-166430-         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression in mycobacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Beckham KSH, Staack S, Wilmanns M, Parret AHA
  TITLE     The pMy vector series: A versatile cloning platform for the 
            recombinant production of mycobacterial proteins in Mycobacterium 
            smegmatis.
  JOURNAL   Protein Sci. 2020 Oct 2. doi: 10.1002/pro.3962.
  PUBMED    33006405
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2020 Oct 2. doi: 10.1002/pro.3962."
COMMENT     Sequence Label: pMyBADNT-kan
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             19..36
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     terminator      166..212
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 caggaggaat taaccatgca ccaccaccac caccacccgt cggccggcga gaacctgtac
       61 ttccagggcg ccatggacga taaggtaccg gatccgaatt cgatatcgct agcaagctta
      121 tcgatgtcga cgtagttaac tagcgtacga tcgactgcca ggcatcaaat aaaacgaaag
      181 gctcagtcga aagactgggc ctttcgtttt atgccatcat ggccgcggtg atcagctaga
      241 gggggcgctg attagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca
      301 ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg
      361 aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca
      421 tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca
      481 tgagtgacga ctgaatccgg tgagaatggc aaaagattat gcatttcttt ccagacttgt
      541 tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc
      601 attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg acaattacaa
      661 acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct
      721 gaatcaggat attcttctaa tacctggaat gctgttttcc cggggatcgc agtggtgagt
      781 aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc
      841 gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca
      901 tgtttcagaa acaactctgg cgcatggggc ttcccataca agcgatagat tgtc
//
