LOCUS       sequence-16644-e        1374 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD96_12
FEATURES             Location/Qualifiers
     source          1..1374
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             358..378
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(421..449)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gnngnnggtc gactctagag gatccccaat tcttgagact tccactatct tctctggcac
       61 cctccactac gcgtaggcgt ctctcgcttc gtgtattccc gggaagccgg gttcccgtct
      121 ctcccgccgc tgccgctgcc gcacacagct ttacacctcg tagaatcccc aaagaggggc
      181 gtggcttgcg ggtgccaaca tcctcctgcc gaggaagaag caggcactca tcactcgcat
      241 catcaacctc gggattggcc aaaggaccca aaggtatgtt tcgaatgata ctaacataac
      301 atagaacatt ttcaggagga cccttggagg gtaccgagct cagaaaaaat gactgctcca
      361 aagaagaagc gtaaggtacc ggtagaaaaa atgagtaaag gagaagaact tttcactgga
      421 gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt
      481 ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact
      541 ggaaaactac ctgttccatg ggtaagttta aacatatata tactaactaa ccctgattat
      601 ttaaattttc agccaacact tgtcactact ttctcttatg gtgttcaatg cttttcaaga
      661 tacccagatc atatgaaacg gcatgacttt ttcaagagtg ccatgcccga aggttatgta
      721 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtaa gtttaaacag
      781 ttcggtacta actaaccata catatttaaa ttttcaggtg ctgaagtcaa gtttgaaggt
      841 gatacccttg ttaatagaat cgagttaaag gtattgattt taaagaagat ggaaacattc
      901 ttggacacaa attggaatac aactataact cacacaatgt atacatcatg gcagacaaac
      961 aaaagaatgg aatcaagttg taagtttaac atgatttact anctaactaa tctgattaan
     1021 tttcnnanct cnaantagac ncacattgaa naggaagcgt tcactagcaa ccntntcaca
     1081 aaatactcca tggggatggc ctgtcnttta ccaaaaacnt nactgtccnc catntgccnt
     1141 tcaanaaccc ncnaaaaana ccntggncct ctganttgna cccgcnggat acntgggngg
     1201 aganttaaaa ngctnntaaa tcccnnggnc ggccncntta cantnttggg naaaaaangg
     1261 ncnccncnan aagttacggg ttcnaaaaga aantttttnn cncgcggncc gaaaaaaaan
     1321 ntnaaccang gtttcngncc cccttttaaa nccttttttt tttnncccaa aaga
//
