LOCUS       sequence-166458-         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression in mycobacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Beckham KSH, Staack S, Wilmanns M, Parret AHA
  TITLE     The pMy vector series: A versatile cloning platform for the 
            recombinant production of mycobacterial proteins in Mycobacterium 
            smegmatis.
  JOURNAL   Protein Sci. 2020 Oct 2. doi: 10.1002/pro.3962.
  PUBMED    33006405
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2020 Oct 2. doi: 10.1002/pro.3962."
COMMENT     Sequence Label: pMyBADC-kan
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(313..359)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(418..435)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(522..806)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(580..599)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 atgcataatc ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
       61 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga
      121 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
      181 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
      241 cagtttcatt tgatgctcga tgagtttttc taatcagcgc cccctctagc tgatcaccgc
      301 ggccatgatg gcataaaacg aaaggcccag tctttcgact gagcctttcg ttttatttga
      361 tgcctggcag tcgatcgtac gctagttaac tacgtcgaca tcgataagct gttatcagtg
      421 gtggtggtgg tggtgcgaag cttggctagc gatatcgaat tcggatccgg taccggccga
      481 gcccatgggg ttaattcctc ctgttagccc aaaaaacggg tatggagaaa cagtagagag
      541 ttgcgataaa aagcgtcagg taggatccgc taatcttatg gataaaaatg ctatggcata
      601 gcaaagtgtg acgccgtgca aataatcaat gtggactttt ctgccgtgat tatagacact
      661 tttgttacgc gtttttgtca tggctttggt cccgctttgt tacagaatgc ttttaataag
      721 cggggttacc ggtttggtta gcgagaagag ccagtaaaag acgcagtgac ggcaatgtct
      781 gatgcaatat ggacaattgg tttcttctct gaatggcggg agtatgaaaa gtatggctga
      841 agcgcaaaat gatcccctgc tgccgggata ctcgtttaat gcccatctgg tggcgggttt
      901 aacgccgatt gaggccaacg gttatctcga
//
