LOCUS       sequence-16646-e        1343 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD96_62
FEATURES             Location/Qualifiers
     source          1..1343
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             256..276
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(319..347)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gngttactga tcttactaac taactattct catttaaatt ttcagagctt aaaaatggct
       61 gaaatcactc acaacgatgg atacgctaac aacttggaaa tgaaataagc ttgcatgcct
      121 gcaggtcgac tctagaggat ccccgggatt ggccaaagga cccaaaggta tgtttcgaat
      181 gatactaaca taacatagaa cattttcagg aggacccttg cttggagggt accgagctca
      241 gaaaaaatga ctgctccaaa gaagaagcgt aaggtaccgg tagaaaaaat gagtaaagga
      301 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg
      361 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt
      421 aaatttattt gcactactgg aaaactacct gttccatggg taagtttaaa catatatata
      481 ctaactaacc ctgattattt aaattttcag ccaacacttg tcactacttt ctgttatggt
      541 gttcaatgct tctcgagata cccagatcat atgaaacggc atgacttttt caagagtgcc
      601 atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag
      661 acacgtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt ttcaggtgct
      721 gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt
      781 aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc acacaatgta
      841 tacatcatgg cagacaaaca aaagaatgga atcaaagttg taagtttaaa catgatttta
      901 ctaactaact aatctgattt aaattttcag aacttcnaaa ttagacacaa cattgaagat
      961 ggaagcgttc actagcagac cattatcacn aaatactcca ttggcgatgg cctgtcnttt
     1021 acnagacanc ntnacctgtc ccacatctgc ccttcgaaga tcccacgaaa naanacnntg
     1081 ncctnctgan ttgnaccctg cgggataccn tgnaggagac taacangncc gggggganac
     1141 naaccccccc acgaccnnaa tgcccgttcn ccctgttggg gancaccccc tttaccccgg
     1201 gggnaacngg gtccatannc ctggaccccc tncccnggna aaaaaccccc cccncccccg
     1261 gngaataaaa cccaattntt anttttnggg ggccntttcc ccnncccnnn ggggggtttn
     1321 nanncccccc cgganaaccc ccc
//
