LOCUS       sequence-166461-         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression in mycobacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Beckham KSH, Staack S, Wilmanns M, Parret AHA
  TITLE     The pMy vector series: A versatile cloning platform for the 
            recombinant production of mycobacterial proteins in Mycobacterium 
            smegmatis.
  JOURNAL   Protein Sci. 2020 Oct 2. doi: 10.1002/pro.3962.
  PUBMED    33006405
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2020 Oct 2. doi: 10.1002/pro.3962."
COMMENT     Sequence Label: pMyBADC
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        330..614
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     537..556
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             701..718
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      777..823
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gcgcggacga aagtaaaccc actggtgata ccattcgcga gcctccggat gacgaccgta
       61 gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc ggtcggcaaa caaattctcg
      121 tccctgattt ttcaccaccc cctgaccgcg aatggtgaga ttgagaatat aacctttcat
      181 tcccagcggt cggtcgataa aaaaatcgag ataaccgttg gcctcaatcg gcgttaaacc
      241 cgccaccaga tgggcattaa acgagtatcc cggcagcagg ggatcatttt gcgcttcagc
      301 catacttttc atactcccgc cattcagaga agaaaccaat tgtccatatt gcatcagaca
      361 ttgccgtcac tgcgtctttt actggctctt ctcgctaacc aaaccggtaa ccccgcttat
      421 taaaagcatt ctgtaacaaa gcgggaccaa agccatgaca aaaacgcgta acaaaagtgt
      481 ctataatcac ggcagaaaag tccacattga ttatttgcac ggcgtcacac tttgctatgc
      541 catagcattt ttatccataa gattagcgga tcctacctga cgctttttat cgcaactctc
      601 tactgtttct ccatacccgt tttttgggct aacaggagga attaacccca tgggctcggc
      661 cggtaccgga tccgaattcg atatcgctag ccaagcttcg caccaccacc accaccactg
      721 ataacagctt atcgatgtcg acgtagttaa ctagcgtacg atcgactgcc aggcatcaaa
      781 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatgccatca tggccgcggt
      841 gatcagctag agggggcgtc aggcgccggg ggcggtgtcc ggcggccccc agaggaa
//
