LOCUS       sequence-166464-        5472 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression in mycobacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5472)
  AUTHORS   Beckham KSH, Staack S, Wilmanns M, Parret AHA
  TITLE     The pMy vector series: A versatile cloning platform for the 
            recombinant production of mycobacterial proteins in Mycobacterium 
            smegmatis.
  JOURNAL   Protein Sci. 2020 Oct 2. doi: 10.1002/pro.3962.
  PUBMED    33006405
REFERENCE   2  (bases 1 to 5472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2020 Oct 2. doi: 10.1002/pro.3962."
COMMENT     Sequence Label: pMyBADNT
FEATURES             Location/Qualifiers
     source          1..5472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(624..1502)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1529..1813
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1736..1755
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             1850..1867
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1880..1900
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     terminator      1997..2043
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      3416..4004
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3905..3924
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgcaaaaag cggcgcgtga ccgccgccga aggcgctcga atcaccggac tatccgaacg
       61 ccacgtcgtc cggctcgtgg cgcaggaacg cagcgagtgg ctcgccgagc aggctgcacg
      121 ccgcgaacgc atccgcgcct atcacgacga cgagggccac tcttggccgc aaacggccaa
      181 acatttcggg ctgcatctgg acaccgttaa gcgactcggc tatcgggcga ggaaagagcg
      241 tgcggcagaa caggaagcgg ctcaaaaggc ccacaacgaa gccgacaatc caccgctgtt
      301 ctaacgcaat tggggagcgg gtgtcgcggg ggttccgtgg ggggttccgt tgcaacgggt
      361 cggacaggta aaagtcctgg tagacgctag ttttctggtt tgggccatgc ctgtctcgtt
      421 gcgtgtttcg ttgcgcccgt tttgaatacc agccagacga gacggggttc tacgaatctt
      481 ggtcgatacc aagccatttc cgctgaatat cggggagctc accgccagaa tcggtggttg
      541 tggtgatgta cgtggcgaac tccgttgtag tgcctgtggt ggcatccgtg gcgcggccgc
      601 ggtaccagat ctttaaatct agattatgac aacttgacgg ctacatcatt cactttttct
      661 tcacaaccgg cacggaactc gctcgggctg gccccggtgc attttttaaa tacccgcgag
      721 aaatagagtt gatcgtcaaa accaacattg cgaccgacgg tggcgatagg catccgggtg
      781 gtgctcaaaa gcagcttcgc ctggctgata cgttggtcct cgcgccagct taagacgcta
      841 atccctaact gctggcggaa aagatgtgac agacgcgacg gcgacaagca aacatgctgt
      901 gcgacgctgg cgatatcaaa attgctgtct gccaggtgat cgctgatgta ctgacaagcc
      961 tcgcgtaccc gattatccat cggtggatgg agcgactcgt taatcgcttc catgcgccgc
     1021 agtaacaatt gctcaagcag atttatcgcc agcagctccg aatagcgccc ttccccttgc
     1081 ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg gctggtgcgc ttcatccggg
     1141 cgaaagaacc ccgtattggc aaatattgac ggccagttaa gccattcatg ccagtaggcg
     1201 cgcggacgaa agtaaaccca ctggtgatac cattcgcgag cctccggatg acgaccgtag
     1261 tgatgaatct ctcctggcgg gaacagcaaa atatcacccg gtcggcaaac aaattctcgt
     1321 ccctgatttt tcaccacccc ctgaccgcga atggtgagat tgagaatata acctttcatt
     1381 cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg cctcaatcgg cgttaaaccc
     1441 gccaccagat gggcattaaa cgagtatccc ggcagcaggg gatcattttg cgcttcagcc
     1501 atacttttca tactcccgcc attcagagaa gaaaccaatt gtccatattg catcagacat
     1561 tgccgtcact gcgtctttta ctggctcttc tcgctaacca aaccggtaac cccgcttatt
     1621 aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa aaacgcgtaa caaaagtgtc
     1681 tataatcacg gcagaaaagt ccacattgat tatttgcacg gcgtcacact ttgctatgcc
     1741 atagcatttt tatccataag attagcggat cctacctgac gctttttatc gcaactctct
     1801 actgtttctc catacccgtt ttttgggcta acaggaggaa ttaaccatgc accaccacca
     1861 ccaccacccg tcggccggcg agaacctgta cttccagggc gccatggacg ataaggtacc
     1921 ggatccgaat tcgatatcgc tagcaagctt atcgatgtcg acgtagttaa ctagcgtacg
     1981 atcgactgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     2041 tatgccatca tggccgcggt gatcagctag agggggcgtc aggcgccggg ggcggtgtcc
     2101 ggcggccccc agaggaactg cgccagttcc tccggatcgg tgaagccgga gagatccagc
     2161 ggggtctcct cgaacacctc gaagtcgtgc aggaaggtga aggcgagcag ttcgcgggcg
     2221 aagtcctcgg tccgcttcca ctgcgccccg tcgagcagcg cggccaggat ctcgcggtcg
     2281 ccccggaagg cgttgagatg cagttgcacc aggctgtagc gggagtctcc cgcatagacg
     2341 tcggtgaagt cgacgatccc ggtgacctcg gtcgcggcca ggtccacgaa gatgttggtc
     2401 ccgtgcaggt cgccgtggac gaaccggggt tcgcggccgg ccagcagcgt gtccacgtcc
     2461 ggcagccagt cctccaggcg gtccagcagc cggggcgaga ggtagcccca cccgcggtgg
     2521 tcctcgacgg tcgccgcgcg gcgttcccgc agcagttccg ggaagacctc ggaatggggg
     2581 gtgagcacgg tgttcccggt cagcggcacc ctgtgcagcc ggccgagcac ccggccgagt
     2641 tcgcgggcca gggcgagcag cgcgttccgg tcggtcgtgc cgtccatcgc ggaccgccag
     2701 gtggtgccgg tcatccggct catcaccagg tagggccacg gccaggctcc ggtgccgggc
     2761 cgcagctcgc cgcggccgag gaggcggggc accggcaccg gggcgtccgc caggaccgcg
     2821 tacgcctccg actccgacgc gaggctctcc ggaccgcacc agtgctcgcc gaacagcttg
     2881 atcaccgggc cgggctcgcc gaccagtacg gggttggtgc tctcgccggg cacccgcagc
     2941 accggcggca ccggcagccc gagctcctcc agggctcggc gggccagcgg ctcccagaat
     3001 tcctggtcgt tccgcaggct cgcgtaggaa tcatccgaat caatacggtc gagaagtaac
     3061 agggattctt gtgtcacagc ggacctctat tcacagggta cgggccggct taattccgca
     3121 cggccggtcg cgacacggcc tgtccgcacc gcggatcagg cgttgacgat gacgggctgg
     3181 tcggccacgt cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     3241 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     3301 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     3361 ctcatgctct agttccatga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     3421 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     3481 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     3541 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     3601 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     3661 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     3721 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     3781 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaggggagaa
     3841 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     3901 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     3961 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     4021 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     4081 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     4141 gccgaacgac cgagcgcaac gcgtgcggcc gcacgcgtga gcccaccagc tccgtaagtt
     4201 cgggcgctgt gtggctcgta cccgcgcatt caggcggcag ggggtctaac gggtctaagg
     4261 cggcgtgtac ggccgccaca gcggctctca gcggcccgga aacgtcctcg aaacgacgca
     4321 tgtgttcctc ctggttggta caggtggttg ggggtgctcg gctgtcgctg gtgttccacc
     4381 accagggctc gacgggagag cgggggagtg tgcagttgtg gggtggcccc tcagcgaaat
     4441 atctgacttg gagctcgtgt cggaccatac accggtgatt aatcgtggtc tactaccaag
     4501 cgtgagccac gtcgccgacg aatttgagca gctctggctg ccgtactggc cgctggcaag
     4561 cgacgatctg ctcgagggga tctaccgcca aagccgcgcg tcggccctag gccgccggta
     4621 catcgaggcg aacccaacag cgctggcaaa cctgctggtc gtggacgtag accatccaga
     4681 cgcagcgctc cgagcgctca gcgcccgggg gtcccatccg ctgcccaacg cgatcgtggg
     4741 caatcgcgcc aacggccacg cacacgcagt gtgggcactc aacgcccctg ttccacgcac
     4801 cgaatacgcg cggcgtaagc cgctcgcata catggcggcg tgcgccgaag gccttcggcg
     4861 cgccgtcgat ggcgaccgca gttactcagg cctcatgacc aaaaaccccg gccacatcgc
     4921 ctgggaaacg gaatggctcc actcagatct ctacacactc agccacatcg aggccgagct
     4981 cggcgcgaac atgccaccgc cgcgctggcg tcagcagacc acgtacaaag cggctccgac
     5041 gccgctaggg cggaattgcg cactgttcga ttccgtcagg ttgtgggcct atcgtcccgc
     5101 cctcatgcgg atctacctgc cgacccggaa cgtggacgga ctcggccgcg cgatctatgc
     5161 cgagtgccac gcgcgaaacg ccgaatttcc gtgcaacgac gtgtgtcccg gaccgctacc
     5221 ggacagcgag gtccgcgcca tcgccaacag catttggcgt tggatcacaa ccaagtcgcg
     5281 catttgggcg gacgggatcg tggtctacga ggccacactc agtgcgcgcc agtcggccat
     5341 ctcgcggaag ggcgcagcag cgcgcacggc ggcgagcaca gttgcgcggc gcgcaaagtc
     5401 cgcgtcagcc atgcatggag gcattgctat gagcgacggc tacagcgacg gctacaaccg
     5461 gcagccgact gt
//
