LOCUS       sequence-166466-        5474 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression in mycobacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5474)
  AUTHORS   Beckham KSH, Staack S, Wilmanns M, Parret AHA
  TITLE     The pMy vector series: A versatile cloning platform for the 
            recombinant production of mycobacterial proteins in Mycobacterium 
            smegmatis.
  JOURNAL   Protein Sci. 2020 Oct 2. doi: 10.1002/pro.3962.
  PUBMED    33006405
REFERENCE   2  (bases 1 to 5474)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2020 Oct 2. doi: 10.1002/pro.3962."
COMMENT     Sequence Label: pMyBADC
FEATURES             Location/Qualifiers
     source          1..5474
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(326..1204)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1231..1515
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1438..1457
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             1602..1619
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      1678..1724
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      3097..3685
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3586..3605
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgtcgcgggg gttccgtggg gggttccgtt gcaacgggtc ggacaggtaa aagtcctggt
       61 agacgctagt tttctggttt gggccatgcc tgtctcgttg cgtgtttcgt tgcgcccgtt
      121 ttgaatacca gccagacgag acggggttct acgaatcttg gtcgatacca agccatttcc
      181 gctgaatatc ggggagctca ccgccagaat cggtggttgt ggtgatgtac gtggcgaact
      241 ccgttgtagt gcctgtggtg gcatccgtgg cgcggccgcg gtaccagatc tttaaatcta
      301 gataaagaag tgacgcggtc ctagattatg acaacttgac ggctacatca ttcacttttt
      361 cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg
      421 agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg
      481 tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc
      541 taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct
      601 gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag
      661 cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc
      721 gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt
      781 gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg
      841 ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg
      901 cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt
      961 agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc
     1021 gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca
     1081 ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac
     1141 ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag
     1201 ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac
     1261 attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta
     1321 ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg
     1381 tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg
     1441 ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct
     1501 ctactgtttc tccatacccg ttttttgggc taacaggagg aattaacccc atgggctcgg
     1561 ccggtaccgg atccgaattc gatatcgcta gccaagcttc gcaccaccac caccaccact
     1621 gataacagct tatcgatgtc gacgtagtta actagcgtac gatcgactgc caggcatcaa
     1681 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatgccatc atggccgcgg
     1741 tgatcagcta gagggggcgt caggcgccgg gggcggtgtc cggcggcccc cagaggaact
     1801 gcgccagttc ctccggatcg gtgaagccgg agagatccag cggggtctcc tcgaacacct
     1861 cgaagtcgtg caggaaggtg aaggcgagca gttcgcgggc gaagtcctcg gtccgcttcc
     1921 actgcgcccc gtcgagcagc gcggccagga tctcgcggtc gccccggaag gcgttgagat
     1981 gcagttgcac caggctgtag cgggagtctc ccgcatagac gtcggtgaag tcgacgatcc
     2041 cggtgacctc ggtcgcggcc aggtccacga agatgttggt cccgtgcagg tcgccgtgga
     2101 cgaaccgggg ttcgcggccg gccagcagcg tgtccacgtc cggcagccag tcctccaggc
     2161 ggtccagcag ccggggcgag aggtagcccc acccgcggtg gtcctcgacg gtcgccgcgc
     2221 ggcgttcccg cagcagttcc gggaagacct cggaatgggg ggtgagcacg gtgttcccgg
     2281 tcagcggcac cctgtgcagc cggccgagca cccggccgag ttcgcgggcc agggcgagca
     2341 gcgcgttccg gtcggtcgtg ccgtccatcg cggaccgcca ggtggtgccg gtcatccggc
     2401 tcatcaccag gtagggccac ggccaggctc cggtgccggg ccgcagctcg ccgcggccga
     2461 ggaggcgggg caccggcacc ggggcgtccg ccaggaccgc gtacgcctcc gactccgacg
     2521 cgaggctctc cggaccgcac cagtgctcgc cgaacagctt gatcaccggg ccgggctcgc
     2581 cgaccagtac ggggttggtg ctctcgccgg gcacccgcag caccggcggc accggcagcc
     2641 cgagctcctc cagggctcgg cgggccagcg gctcccagaa ttcctggtcg ttccgcaggc
     2701 tcgcgtagga atcatccgaa tcaatacggt cgagaagtaa cagggattct tgtgtcacag
     2761 cggacctcta ttcacagggt acgggccggc ttaattccgc acggccggtc gcgacacggc
     2821 ctgtccgcac cgcggatcag gcgttgacga tgacgggctg gtcggccacg tcggggagtc
     2881 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     2941 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     3001 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgctc tagttccatg
     3061 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3121 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3181 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3241 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3301 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3361 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3421 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3481 gcgtgagcta tgagaaagcg ccacgcttcc cgaggggaga aaggcggaca ggtatccggt
     3541 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     3601 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     3661 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     3721 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     3781 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcaa
     3841 cgcgtgcggc cgcacgcgtg agcccaccag ctccgtaagt tcgggcgctg tgtggctcgt
     3901 acccgcgcat tcaggcggca gggggtctaa cgggtctaag gcggcgtgta cggccgccac
     3961 agcggctctc agcggcccgg aaacgtcctc gaaacgacgc atgtgttcct cctggttggt
     4021 acaggtggtt gggggtgctc ggctgtcgct ggtgttccac caccagggct cgacgggaga
     4081 gcgggggagt gtgcagttgt ggggtggccc ctcagcgaaa tatctgactt ggagctcgtg
     4141 tcggaccata caccggtgat taatcgtggt ctactaccaa gcgtgagcca cgtcgccgac
     4201 gaatttgagc agctctggct gccgtactgg ccgctggcaa gcgacgatct gctcgagggg
     4261 atctaccgcc aaagccgcgc gtcggcccta ggccgccggt acatcgaggc gaacccaaca
     4321 gcgctggcaa acctgctggt cgtggacgta gaccatccag acgcagcgct ccgagcgctc
     4381 agcgcccggg ggtcccatcc gctgcccaac gcgatcgtgg gcaatcgcgc caacggccac
     4441 gcacacgcag tgtgggcact caacgcccct gttccacgca ccgaatacgc gcggcgtaag
     4501 ccgctcgcat acatggcggc gtgcgccgaa ggccttcggc gcgccgtcga tggcgaccgc
     4561 agttactcag gcctcatgac caaaaacccc ggccacatcg cctgggaaac ggaatggctc
     4621 cactcagatc tctacacact cagccacatc gaggccgagc tcggcgcgaa catgccaccg
     4681 ccgcgctggc gtcagcagac cacgtacaaa gcggctccga cgccgctagg gcggaattgc
     4741 gcactgttcg attccgtcag gttgtgggcc tatcgtcccg ccctcatgcg gatctacctg
     4801 ccgacccgga acgtggacgg actcggccgc gcgatctatg ccgagtgcca cgcgcgaaac
     4861 gccgaatttc cgtgcaacga cgtgtgtccc ggaccgctac cggacagcga ggtccgcgcc
     4921 atcgccaaca gcatttggcg ttggatcaca accaagtcgc gcatttgggc ggacgggatc
     4981 gtggtctacg aggccacact cagtgcgcgc cagtcggcca tctcgcggaa gggcgcagca
     5041 gcgcgcacgg cggcgagcac agttgcgcgg cgcgcaaagt ccgcgtcagc catgcatgga
     5101 ggcattgcta tgagcgacgg ctacagcgac ggctacaacc ggcagccgac tgtccgcaaa
     5161 aagcggcgcg tgaccgccgc cgaaggcgct cgaatcaccg gactatccga acgccacgtc
     5221 gtccggctcg tggcgcagga acgcagcgag tggctcgccg agcaggctgc acgccgcgaa
     5281 cgcatccgcg cctatcacga cgacgagggc cactcttggc cgcaaacggc caaacatttc
     5341 gggctgcatc tggacaccgt taagcgactc ggctatcggg cgaggaaaga gcgtgcggca
     5401 gaacaggaag cggctcaaaa ggcccacaac gaagccgaca atccaccgct gttctaacgc
     5461 aattggggag cggg
//
