LOCUS       sequence-166468-        4005 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing PTEN, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_PTEN_p.G127R
FEATURES             Location/Qualifiers
     source          1..4005
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      36..624
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     525..544
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     778..795
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(954..981)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1073..1159)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1208..1230
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1222..1239
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1223..1239
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1255..1354
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2570..2669)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2686..2705)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2687..2705)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2710..2726)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2710..2726)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3157..3948
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
       61 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
      121 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
      181 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
      241 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
      301 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
      361 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
      421 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
      481 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
      541 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
      601 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
      661 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
      721 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
      781 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
      841 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
      901 cgcaacgcaa ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc
      961 catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg
     1021 cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc
     1081 tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact
     1141 gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg
     1201 atgttttccc agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gccccaaata
     1261 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt
     1321 tttataatgc caactttgta caaaaaagtt ggcaccatga cagccatcat caaagagatc
     1381 gttagcagaa acaaaaggag atatcaagag gatggattcg acttagactt gacctatatt
     1441 tatccaaaca ttattgctat gggatttcct gcagaaagac ttgaaggcgt atacaggaac
     1501 aatattgatg atgtagtaag gtttttggat tcaaagcata aaaaccatta caagatatac
     1561 aatctttgtg ctgaaagaca ttatgacacc gccaaattta attgcagagt tgcacaatat
     1621 ccttttgaag accataaccc accacagcta gaacttatca aacccttttg tgaagatctt
     1681 gaccaatggc taagtgaaga tgacaatcat gttgcagcaa ttcactgtaa agctagaaag
     1741 ggacgaactg gtgtaatgat atgtgcatat ttattacatc ggggcaaatt tttaaaggca
     1801 caagaggccc tagatttcta tggggaagta aggaccagag acaaaaaggg agtaactatt
     1861 cccagtcaga ggcgctatgt gtattattat agctacctgt taaagaatca tctggattat
     1921 agaccagtgg cactgttgtt tcacaagatg atgtttgaaa ctattccaat gttcagtggc
     1981 ggaacttgca atcctcagtt tgtggtctgc cagctaaagg tgaagatata ttcctccaat
     2041 tcaggaccca cacgacggga agacaagttc atgtactttg agttccctca gccgttacct
     2101 gtgtgtggtg atatcaaagt agagttcttc cacaaacaga acaagatgct aaaaaaggac
     2161 aaaatgtttc acttttgggt aaatacattc ttcataccag gaccagagga aacctcagaa
     2221 aaagtagaaa atggaagtct atgtgatcaa gaaatcgata gcatttgcag tatagagcgt
     2281 gcagataatg acaaggaata tctagtactt actttaacaa aaaatgatct tgacaaagca
     2341 aataaagaca aagccaaccg atacttttct ccaaatttta aggtgaagct gtacttcaca
     2401 aaaacagtag aggagccgtc aaatccagag gctagcagtt caacttctgt aacaccagat
     2461 gttagtgaca atgaacctga tcattataga tattctgaca ccactgactc tgatccagag
     2521 aatgaacctt ttgatgaaga tcagcataca caaattacaa aagtctgaga cccagctttc
     2581 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
     2641 actatcagtc aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg
     2701 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt
     2761 tacattgcac aagataaaaa tatatcatca tgcctcctct agaccagcca ggacagaaat
     2821 gcctcgactt cgctgctacc caaggttgcc gggtgacgca caccgtggaa acggatgaag
     2881 gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg
     2941 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
     3001 gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag
     3061 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
     3121 cagcagggca gtcgccctaa aacaaagtta aacattatga gggaagcggt gatcgccgaa
     3181 gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg
     3241 ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt
     3301 gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac
     3361 gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc
     3421 accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa
     3481 tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac
     3541 attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca
     3601 gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa
     3661 accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt
     3721 acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct
     3781 gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga
     3841 caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa
     3901 tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc ctcgagccac
     3961 ccatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actga
//
