LOCUS       sequence-166469-        1250 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses DNA-binding (ETS) domain of murine Ets-1, residues 311 to 
            440.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Wang S, Linde MH, Munde M, Carvalho VD, Wilson WD, Poon GM
  TITLE     Mechanistic heterogeneity in site recognition by the structurally 
            homologous DNA-binding domains of the ETS family transcription 
            factors Ets-1 and PU.1.
  JOURNAL   J Biol Chem. 2014 Aug 1;289(31):21605-16. doi: 
            10.1074/jbc.M114.575340. Epub 2014 Jun 21.
  PUBMED    24952944
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.575340"; journalName: "J Biol Chem"; date: 
            "2014-08-1- 1"; volume: "289"; issue: "31"; pages: "21605-16"
COMMENT     Sequence Label: pET28b-Ets1-ETS
FEATURES             Location/Qualifiers
     source          1..1250
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             388..405
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             406..423
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             433..450
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(503..521)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      517..564
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      601..1056
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(688..707)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     898..919
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtggcgaatt cctctagaat aattttgttt actttaagaa ggagatatac catgggcagt
       61 ggaccaatcc agctatggca gtttcttctg gaattactca ctgataaatc ctgtcagtct
      121 tttatcagct ggacaggaga tggctgggaa ttcaaacttt ctgacccaga tgaggtggcc
      181 aggagatggg gaaagaggaa aaacaaacct aagatgaatt atgagaagct gagccgtggc
      241 ctacgctact attacgacaa aaacatcatc cacaagacag cggggaaacg ctacgtgtac
      301 cgctttgtgt gtgacctgca gagcctgctg gggtacaccc ctgaggagct gcacgccatg
      361 ctggacgtca agccagatgc cgacgagcta gtgccacgag gttcccatca ccatcaccat
      421 cactaactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag
      481 gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct
      541 aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatt ggcgaatggg
      601 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
      661 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      721 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      781 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      841 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      901 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      961 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     1021 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     1081 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgaa
     1141 ttaattctta gaaaaactca tcgagcatca aatgaaactg ccatttattc ttttcaggat
     1201 atccataccc tattttttaa aaaaccgttt ctgtaatgaa ggagaaaaat
//
