LOCUS       sequence-166477-         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically 
            selected. Modified from BBa_R0040. [G:36INS-pTet:B].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Carr SB, Beal J, Densmore DM
  TITLE     Reducing DNA context dependence in bacterial promoters.
  JOURNAL   PLoS One. 2017 Apr 19;12(4):e0176013. doi: 
            10.1371/journal.pone.0176013. eCollection 2017.
  PUBMED    28422998
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2017-04-19"; volume: "12(4):e0176013. doi"; pages: " 
            10.1371/journal.pone.0176013. eCollection 2017"
COMMENT     Sequence Label: 36INS-pTet_GB
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    22..40
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    47..65
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    120..141
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      144..187
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(191..209)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        277..381
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(600..619)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ggtctctagt agtgctcagt atctctatca ctgataggga tgtcaatctc tatcactgat
       61 agggaacaac agtgaggttt ctttactatg tattctctcc gggcatgaga cccactagtc
      121 tctagaagcg gccgcgaatt ccagaaatca tccttagcga aagctaagga ttttttttat
      181 ctgaaattct gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
      241 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
      301 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
      361 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
      421 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
      481 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
      541 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
      601 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
      661 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
      721 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
      781 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
      841 ggggatcatg taactcgcct tgatcgttgg ga
//
