LOCUS       sequence-166482-        3435 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing VHL, part of the Target Accelerator 
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_VHL_p.V130F
FEATURES             Location/Qualifiers
     source          1..3435
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      46..634
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     535..554
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     788..805
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(964..991)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1083..1169)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1218..1240
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1232..1249
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1233..1249
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1265..1364
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2010..2109)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2126..2145)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2127..2145)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2150..2166)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2150..2166)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2597..3388
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
       61 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
      121 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
      181 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
      241 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
      301 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
      361 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
      421 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
      481 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
      541 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
      601 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
      661 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
      721 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
      781 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
      841 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
      901 gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc caggaagagt ttgtagaaac
      961 gcaaaaaggc catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc
     1021 gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc
     1081 ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag
     1141 tcttccgact gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac
     1201 gctagcatgg atgttttccc agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg
     1261 gccccaaata atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga
     1321 gcaatgcttt tttataatgc caactttgta caaaaaagtt ggcaccatgc cccggagggc
     1381 ggagaactgg gacgaggccg aggtaggcgc ggaggaggca ggcgtcgaag agtacggccc
     1441 tgaagaagac ggcggggagg agtcgggcgc cgaggagtcc ggcccggaag agtccggccc
     1501 ggaggaactg ggcgccgagg aggagatgga ggccgggcgg ccgcggcccg tgctgcgctc
     1561 ggtgaactcg cgcgagccct cccaggtcat cttctgcaat cgcagtccgc gcgtcgtgct
     1621 gcccgtatgg ctcaacttcg acggcgagcc gcagccctac ccaacgctgc cgcctggcac
     1681 gggccgccgc atccacagct accgaggtca cctttggctc ttcagagatg cagggacaca
     1741 cgatgggctt ctgtttaacc aaactgaatt atttgtgcca tctctcaatg ttgacggaca
     1801 gcctattttt gccaatatca cactgccagt gtatactctg aaagagcgat gcctccaggt
     1861 tgtccggagc ctagtcaagc ctgagaatta caggagactg gacatcgtca ggtcgctcta
     1921 cgaagatctg gaagaccacc caaatgtgca gaaagacctg gagcggctga cacaggagcg
     1981 cattgcacat caacggatgg gagattgatt gccaactttc ttgtacaaag ttggcattat
     2041 aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
     2101 cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt
     2161 ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
     2221 tatatcatca tgcctcctct agaccagcca ggacagaaat gcctcgactt cgctgctacc
     2281 caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc agtggacata
     2341 agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag
     2401 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
     2461 ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt
     2521 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa
     2581 aacaaagtta aacattatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga
     2641 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg
     2701 ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac
     2761 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc
     2821 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga
     2881 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa
     2941 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct
     3001 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga
     3061 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc
     3121 gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta
     3181 cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg
     3241 cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga
     3301 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg
     3361 cgagatcacc aaggtagtcg gcaaataacc ctcgagccac ccatgaccaa aatcccttaa
     3421 cgtgagttac gcgtc
//
