LOCUS       sequence-166484-        4005 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing PTEN, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_PTEN_p.R233Q
FEATURES             Location/Qualifiers
     source          1..4005
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      17..605
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     506..525
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     759..776
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(935..962)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1054..1140)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1189..1211
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1203..1220
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1204..1220
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1236..1335
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2551..2650)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2667..2686)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2668..2686)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2691..2707)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2691..2707)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3138..3929
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
       61 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      121 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      181 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      241 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      301 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      361 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      421 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      481 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      541 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      601 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      661 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
      721 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      781 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
      841 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatacgc
      901 gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt
      961 ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg
     1021 ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac
     1081 cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc gttttatttg
     1141 atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac
     1201 gttgtaaaac gacggccagt cttaagctcg ggccccaaat aatgatttta ttttgactga
     1261 tagtgacctg ttcgttgcaa caaattgatg agcaatgctt ttttataatg ccaactttgt
     1321 acaaaaaagc aggcaccatg acagccatca tcaaagagat cgttagcaga aacaaaagga
     1381 gatatcaaga ggatggattc gacttagact tgacctatat ttatccaaac attattgcta
     1441 tgggatttcc tgcagaaaga cttgaaggcg tatacaggaa caatattgat gatgtagtaa
     1501 ggtttttgga ttcaaagcat aaaaaccatt acaagatata caatctttgt gctgaaagac
     1561 attatgacac cgccaaattt aattgcagag ttgcacaata tccttttgaa gaccataacc
     1621 caccacagct agaacttatc aaaccctttt gtgaagatct tgaccaatgg ctaagtgaag
     1681 atgacaatca tgttgcagca attcactgta aagctggaaa gggacgaact ggtgtaatga
     1741 tatgtgcata tttattacat cggggcaaat ttttaaaggc acaagaggcc ctagatttct
     1801 atggggaagt aaggaccaga gacaaaaagg gagtaactat tcccagtcag aggcgctatg
     1861 tgtattatta tagctacctg ttaaagaatc atctggatta tagaccagtg gcactgttgt
     1921 ttcacaagat gatgtttgaa actattccaa tgttcagtgg cggaacttgc aatcctcagt
     1981 ttgtggtctg ccagctaaag gtgaagatat attcctccaa ttcaggaccc acacaacggg
     2041 aagacaagtt catgtacttt gagttccctc agccgttacc tgtgtgtggt gatatcaaag
     2101 tagagttctt ccacaaacag aacaagatgc taaaaaagga caaaatgttt cacttttggg
     2161 taaatacatt cttcatacca ggaccagagg aaacctcaga aaaagtagaa aatggaagtc
     2221 tatgtgatca agaaatcgat agcatttgca gtatagagcg tgcagataat gacaaggaat
     2281 atctagtact tactttaaca aaaaatgatc ttgacaaagc aaataaagac aaagccaacc
     2341 gatacttttc tccaaatttt aaggtgaagc tgtacttcac aaaaacagta gaggagccgt
     2401 caaatccaga ggctagcagt tcaacttctg taacaccaga tgttagtgac aatgaacctg
     2461 atcattatag atattctgac accactgact ctgatccaga gaatgaacct tttgatgaag
     2521 atcagcatac acaaattaca aaagtctgat tgccaacttt cttgtacaaa gttggcatta
     2581 taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa
     2641 tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg
     2701 tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa
     2761 atatatcatc atgcctcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctac
     2821 ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat
     2881 aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca
     2941 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
     3001 actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
     3061 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
     3121 aaacaaagtt aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag
     3181 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
     3241 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga
     3301 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
     3361 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
     3421 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
     3481 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
     3541 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
     3601 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
     3661 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
     3721 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
     3781 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag
     3841 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag
     3901 gcgagatcac caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta
     3961 acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaa
//
