LOCUS       sequence-16649-e        1391 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD61_125
FEATURES             Location/Qualifiers
     source          1..1391
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..66
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     50..66
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(136..152)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(137..153)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(177..196)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(178..196)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(206..223)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(206..222)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(215..237)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      364..819
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(451..470)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     661..682
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        845..949
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gatacctact aagggacaaa agctggagct ccaccgcggt ggcggccgct ctagaactag
       61 tggatccacc ggttccatgg ctagccacgt gacgcgtgga tcccccgggc tgcaggaatt
      121 cgatatcaag cttatcgata ccgtcgacct cgaggggggg cccggtaccc aattcgccct
      181 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
      241 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
      301 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
      361 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
      421 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      481 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      541 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      601 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      661 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      721 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      781 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
      841 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
      901 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
      961 acatttccgt gtcgccctta attccttttt tgcggcattt tngcccttcc tgnttttgct
     1021 cacccaaaaa cgctggngaa aagtaaaaan gctgaaaana anttgggtgc acnannggnt
     1081 naatncnaan tggatntcaa agcggnaaaa tccttgaaan tttncccccn aaaaaagttt
     1141 tccaaggaan aacnntttaa ngttngctat gngggnggaa taanccgnat gancccggna
     1201 aaaaaaatcg gggccncaaa natatttcca aaagantggg ggnaaacccc cccccaaaaa
     1261 ncntnnngag gggggaaaaa aaaaatngcn gggncccanc aaagaaaanc ggggccnntt
     1321 tttaaaaatg ggaaaaaaaa aacctttttt aaaagggaaa aatccttttn gggaaaaaaa
     1381 aaaaaaaaan a
//
