LOCUS       sequence-16650-e        1363 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 1363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD95_15
FEATURES             Location/Qualifiers
     source          1..1363
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    26..133
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     42..58
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     43..59
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(93..109)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(93..109)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(141..160)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(142..160)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(167..184)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(167..183)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(176..198)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      325..780
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(412..431)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     622..643
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        806..911
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tanctcacta aagggaacaa aagctggtac cgggcccccc ctcgaggtcg acggtatcga
       61 taagcttgat atcgaattcc tgcagcccgg gggatccact agttctagag cggccgccac
      121 cgcggtggag ctccaattcg ccctatagtg agtcgtatta caattcactg gccgtcgttt
      181 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
      241 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
      301 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
      361 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
      421 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
      481 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
      541 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
      601 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
      661 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
      721 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
      781 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
      841 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      901 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt ttttgcggca
      961 ttttgccttc ctgtttttgc tcacccanaa aacgctggtg aaaagtaaaa gangctgaaa
     1021 aatcagttgg gtgcacgang ngggttanat cgaantggat ctcaaaagcg gtaaanatcc
     1081 ttganagttt tccccccgaa aaangttttc caangangaa cacttttaan gttctgctat
     1141 gngggggngg tattancccn naatngacnc cgggnaaaaa aaanntnggn cncncnanaa
     1201 ctatttncaa aaaganttgg ntgaaaaccc cccagtnaaa aaaaaactnt tagggagggn
     1261 tgaaanaaaa aaaatntgcn ggggtgcnta accggaagaa aaaanggggg ccaattnttt
     1321 tnaaaaatgg ggnaaaaaaa aaaccntttt tcnaangggg aaa
//
