LOCUS       sequence-166513-        3363 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing NRAS, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_NRAS_p.G12C
FEATURES             Location/Qualifiers
     source          1..3363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      35..623
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     524..543
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     777..794
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(953..980)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1072..1158)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1207..1229
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1221..1238
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1222..1238
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1254..1353
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1927..2026)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2043..2062)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2044..2062)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2067..2083)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2067..2083)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2514..3305
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
       61 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
      121 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
      181 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
      241 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
      301 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
      361 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
      421 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
      481 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
      541 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
      601 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
      661 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
      721 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
      781 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
      841 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
      901 gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc
      961 atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc
     1021 ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct
     1081 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg
     1141 agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg ctagcatgga
     1201 tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg ccccaaataa
     1261 tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgatgag caatgctttt
     1321 ttataatgmc aactttgtac aaaaaagttg gcaccatgac tgagtacaaa ctggtggtgg
     1381 ttggagcatg tggtgttggg aaaagcgcac tgacaatcca gctaatccag aaccactttg
     1441 tagatgaata tgatcccacc atagaggatt cttacagaaa acaagtggtt atagatggtg
     1501 aaacctgttt gttggacata ctggatacag ctggacaaga agagtacagt gccatgagag
     1561 accaatacat gaggacaggc gaaggcttcc tctgtgtatt tgccatcaat aatagcaagt
     1621 catttgcgga tattaacctc tacagggagc agattaagcg agtaaaagac tcggatgatg
     1681 tacctatggt gctagtggga aacaagtgtg atttgccaac aaggacagtt gatacaaaac
     1741 aagcccacga actggccaag agttacggga ttccattcat tgaaacctca gccaagacca
     1801 gacagggtgt tgaagatgct ttttacacac tggtaagaga aatacgccag taccgaatga
     1861 aaaaactcaa cagcagtgat gatgggactc agggttgtat gggattgcca tgtgtggtga
     1921 tgtaattgcc aactttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt
     1981 gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata
     2041 tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt
     2101 ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatgc ctcctctaga
     2161 ccagccagga cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac
     2221 cgtggaaacg gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt
     2281 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
     2341 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta
     2401 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
     2461 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg
     2521 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
     2581 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
     2641 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
     2701 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
     2761 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
     2821 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
     2881 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
     2941 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
     3001 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
     3061 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
     3121 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
     3181 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
     3241 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca
     3301 aataaccctc gagccaccca tgaccaaaat cccttaacgt gagttacgcg tcgttccact
     3361 gag
//
