LOCUS       sequence-16652-e        1330 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L2603
FEATURES             Location/Qualifiers
     source          1..1330
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             624..644
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(687..715)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gnncttacta actaactatt ctcatttaaa ttttcagagc ttaaaaatgg ctgancccac
       61 tcacaacgat ggatacgcta acaacttgga aatgaaataa gcttgcatgc ctgcaggcct
      121 tggtcgactc tagaggatcc ccggggattg gccaaaggac ccaaaggtat gtttcgaatg
      181 atactaacat aacatagaac attttcagga ggacccttgc ttggctagca aaaatgcata
      241 aggttttgct ggcactgttc tttatctttc tggcaccagc atccgcactg gcagtctccg
      301 aaccggcctg caggtcgatt ttttgcaaat tacgagcgtt gtagggggcg gagcgatagg
      361 tcctataggt tttggtatat catcattcat tcattcattg gtacattcat ttacccacct
      421 tcctctttct gagcttctct ggagttctgt gcttcctttt tcccttatct ttatactgta
      481 atttttaact ttcaggcatt gattggatcc ccgggattgg ccaaaggacc caaaggtatg
      541 tttcgaatga tactaacata acatagaaca ttttcaggag gacccttgct tggagggtac
      601 cgagctcaga aaaaatgact gctccaaaga agaagcgtaa ggtaccggta gaaaaaatga
      661 gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg
      721 ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac
      781 ttacccttaa atttatttgc actactggaa aactacctgt tccatgggta agtttaaaca
      841 tatatatact aactaaccct gattatttaa attttcagcc aacacttgtc actactttct
      901 gttatggtgn tcaatgcttc tcgaganacc cagatcatat gaaacggcat gactttttca
      961 agagngccat gcccgaaggn tatgtacagg aaagaactat atttttcaaa aatgacggga
     1021 nctacaaaac acgtangttt aaacagtccg gactanctan cntacatatt taantttcag
     1081 gggctgaagt canttngaag ggannccttg tnaaaaancn agtaaangna tgatttnaaa
     1141 aaaggnaant nctggacnna ttggaaacnc ttacncnnna ggttaccctn ggnaaaanaa
     1201 aaaggnacca ggggagttaa cggattncnc aacaancgat nanttcnanc caaaaaaccn
     1261 ctggangnng ntcnncnaac atnaaaaacc ctngggggcg ntttnaaaaa aatgccaang
     1321 gcttaaanaa
//
