LOCUS       sequence-166527-       12072 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression vector for V5-NLGN2 in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12072)
  AUTHORS   Loh KH, Stawski PS, Draycott AS, Udeshi ND, Lehrman EK, Wilton DK, 
            Svinkina T, Deerinck TJ, Ellisman MH, Stevens B, Carr SA, Ting AY
  TITLE     Proteomic Analysis of Unbounded Cellular Compartments: Synaptic 
            Clefts.
  JOURNAL   Cell. 2016 Aug 25;166(5):1295-1307.e21. doi: 
            10.1016/j.cell.2016.07.041.
  PUBMED    27565350
REFERENCE   2  (bases 1 to 12072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.07"; journalName: "Cell"; date: "2016-08-25- 
            25"; volume: "166"; issue: "5"; pages: "1295-1307.e21"
COMMENT     Sequence Label: FSW-HRP-V5-NLGN2
FEATURES             Location/Qualifiers
     source          1..12072
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      258..267
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1350..1391
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3907..4495
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3960..3980)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4378..4389)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4498..4514)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4499..4515)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5020..5200
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5226..5243)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5232..5456
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5502..5930
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5589..5608)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5799..5820
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5939..5959)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5944..6273
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6124..6259
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6186..6205
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6321..6368
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6387..6761
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6891..7012
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6928..6947)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6982..7001
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7061..7077)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7061..7077)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7074..7096)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7085..7101
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7109..7115,7116..7133,7134..7139))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7154..7175
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7292..7309)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7463..8051)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7543..7562)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8222..9013,9014..9082))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8845..8864
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9083..9187)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9262..9281)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9453..9832
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9834..10032
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9987..10007
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             10050..10230
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10277..10402
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10895..11128
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11313..11357
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11655..11772
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(11851..12072,1..226)
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
ORIGIN
        1 cctcagcact gaaggcgcgc tgacgtcact cgccggtccc ccgcaaactc cccttcccgg
       61 ccaccttggt cgcgtccgcg ccgccgccgg cccagccgga ccgcaccacg cgaggcgcga
      121 gatagggggg cacgggcgcg accatctgcg ctgcggcgcc ggcgactcag cgctgcctca
      181 gtctgcggtg ggcagcggag gagtcgtgtc gtgcctgaga gcgcagtcta gaggatcctt
      241 cgaaaccggt gctagccgcc accatggcac ttcccagatg catgtggcca aattatgttt
      301 ggagagctat gatggcatgt gtggtccaca ggggatccgg tgccccattg actctctgct
      361 tgttgggatg tttgctacag acttttcacg tactctctca aaagaccggt cagcttactc
      421 caactttcta cgataactct tgtcctaacg tgagcaacat cgtgcgagat actatcgtga
      481 acgagttgcg gtctgatccg aggatagccg cttcaatttt gcggctgcat ttccacgatt
      541 gttttgtaaa cggctgtgat gcttccatcc tcctcgacaa taccacctct ttcaggactg
      601 aaaaggatgc cttcggtaac gcgaatagtg ctagagggtt tcccgtgatc gatagaatga
      661 aagccgctgt ggagagcgcc tgcccccgga cagttagttg tgcagacctt ctgaccatag
      721 cggcccagca atccgtgacg ctggccggtg gccccagctg gagggtcccc ctgggaagga
      781 gggactctct gcaggctttt ctggacttgg caaacgcaaa cctgcctgct cccttcttca
      841 cccttcccca gctgaaggat tccttcagga atgtcggcct gaaccgaagt agtgatttgg
      901 tggctctgtc cggaggccac acattcggca agaatcagtg caggttcatc atggacagac
      961 tgtataactt ttccaacacc ggcttgcctg accctaccct gaacacaaca tacttgcaga
     1021 ctctcagagg gctgtgtccg ctcaatggca acttgtctgc actggttgac tttgacctgc
     1081 gcaccccaac catcttcgac aataagtatt acgtcaatct ggaggaacag aagggcctga
     1141 ttcagtccga ccaggagctg ttcagttccc caaatgctac cgacactatt cctcttgtgc
     1201 gcagcttcgc taatagcaca caaacctttt tcaatgcttt cgtggaagct atggatcgga
     1261 tgggaaatat aacgcccctg acgggtacgc agggacagat aagactgaac tgtagggtcg
     1321 tgaacagcaa ctctggctcg ggctcgggcg gcaagcccat ccccaacccc ctgctgggcc
     1381 tggacagcac ccaattgcaa cggggaggag ggggtcccgg cggcggcgcc ccgggcggcc
     1441 caggcctggg cctcggcagc ctcggggagg agcgcttccc cgtggtgaac acagcctacg
     1501 ggcgagtgcg cggtgtgcgg cgcgagctca acaacgagat cctgggcccg gtcgtgcagt
     1561 tcttgggcgt gccctacgcc acgccgccct tgggcgcccg ccgcttccag ccgcctgagg
     1621 cacctgcctc gtggcccggc gtgcgcaacg ccaccaccct gccgcccgcc tgcccgcaga
     1681 acctgcacgg ggccctgccg gccatcatgc tgcctgtgtg gttcaccgac aacttggagg
     1741 cggccgccac ctacgtgcag aaccagagcg aggactgcct gtacctcaac ctctacgtgc
     1801 ccactgagga cggtccgctc acaaaaaaac gtgacgaggc gacgctcaat ccgccagaca
     1861 cagatatccg ggactctggg aagaaaccgg tcatgctgtt tctacacggc ggctcctaca
     1921 tggaaggcac cgggaacatg tttgacggct cagtcctggc tgcctatggc aatgtcatcg
     1981 tagccacact caactaccgt cttggggtgc tcggttttct cagcactggt gaccaggctg
     2041 caaaaggcaa ctatgggctc ctggaccaga tccaggccct gcgctggctc agtgaaaaca
     2101 ttgcccactt tggaggtgac cctgaacgca tcactatctt tgggtctggt gcaggggcct
     2161 cctgtgtcaa cttgctgatc ctttcccacc actcagaagg actgttccag aaggccattg
     2221 ctcaaagtgg tactgccatt tccagctggt ctgtcaacta ccagccgctc aagtacacgc
     2281 ggctgctggc ggccaaagtg ggctgtgacc gagaagacag cactgaagct gtggagtgtc
     2341 tgcgccggaa gtcttcccgg gagctagtgg accaggatgt acagcctgcc cgctaccaca
     2401 ttgcctttgg gcctgtggtg gacggcgacg tagtccctga tgaccccgag atcctcatgc
     2461 aacaggggga attcctcaac tacgacatgc tcattggtgt caaccaggga gagggtctca
     2521 agttcgtgga ggactctgca gagagtgagg acggtgtgtc tgccagcgcc tttgacttca
     2581 ccgtctccaa ctttgtggac aacttgtacg ggtacccaga aggcaaggac gtgcttcgag
     2641 agaccatcaa gttcatgtac acggactggg ctgacaggga caatggcgag atgcggcgga
     2701 agaccctgct ggcgctcttt accgaccacc agtgggtcgc cccggctgtg gccaccgcca
     2761 agctgcatgc cgactaccag tcccccgtct acttttacac tttctaccac cactgccagg
     2821 cagagggccg gccagagtgg gcagacgcag cgcacgggga cgagctgccc tacgtctttg
     2881 gtgtccccat ggtgggcgcc actgacctct tcccctgcaa cttctccaag aacgatgtca
     2941 tgctcagcgc agtagtcatg acctactgga ccaacttcgc caagactggt gaccccaacc
     3001 agcctgtgcc acaggacacc aagttcatcc acaccaagcc caaccgcttt gaagaggtag
     3061 tgtggagcaa gttcaacagc aaggaaaagc agtatctgca cataggcttg aaaccacgcg
     3121 tgcgcgacaa ctaccgtgcc aacaaggtgg ccttctggct ggagctcgtg ccccacctgc
     3181 acaacctgca cacagagctc ttcaccacca ccactcgcct gcctccctat gccacacgct
     3241 ggccacctcg cacacctggt cctggcactt ccggcacacg ccgtcctccc ccacctgcca
     3301 ctctgccacc tgagtctgat attgacctag gcccaagggc ctatgaccgc ttccccggtg
     3361 actcgaggga ctactccacg gagctaagcg tgactgtggc agtgggtgcc tccctcctct
     3421 tcctcaacat ccttgccttt gccgccctct attacaagcg ggaccggcgc caggagctgc
     3481 ggtgccggag gcttagccca ccaggaggct caggctcagg tgtgcctggt gggggccccc
     3541 tgcttcccac tgctggccgt gagctacccc cggaggagga gctagtatcg ctgcagctga
     3601 agcggggtgg tggtgttggg gcggaccctg ctgaggccct gcgccctgcc tgtccacccg
     3661 actataccct ggccttgcgc cgggcaccgg acgatgtgcc tctcttggcc cccggggccc
     3721 taaccctgct gcctagtggc ctggggcccc cgcccccgcc cccaccccct tctctccatc
     3781 cctttgggcc cttcccaccc ccacccccta ctgctaccag ccacaacaac acgctacccc
     3841 atccccactc caccactcgg gtatagggcg cgcccctgca gggaattcga tatcaagctt
     3901 atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     3961 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     4021 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     4081 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     4141 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     4201 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     4261 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     4321 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     4381 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     4441 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga
     4501 taccgtcgac ctcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct
     4561 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
     4621 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     4681 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt
     4741 gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg
     4801 ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa
     4861 gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg
     4921 catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca
     4981 tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag
     5041 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     5101 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     5161 tccctcagac ccttttagtc agtgtggaaa atctctagca gggcccgttt aaacccgctg
     5221 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     5281 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     5341 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     5401 gggggaggat tgggaagaca atagcaggca tgctggggat gcggcgggct ctatggcttc
     5461 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     5521 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     5581 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     5641 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     5701 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     5761 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     5821 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     5881 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     5941 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     6001 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     6061 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     6121 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     6181 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     6241 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     6301 ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat
     6361 aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca
     6421 ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg
     6481 acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg
     6541 cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg
     6601 acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc
     6661 cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg
     6721 ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg
     6781 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     6841 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
     6901 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     6961 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     7021 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     7081 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     7141 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     7201 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     7261 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     7321 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     7381 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     7441 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     7501 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     7561 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     7621 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     7681 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     7741 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     7801 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     7861 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     7921 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     7981 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     8041 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     8101 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     8161 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     8221 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     8281 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     8341 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     8401 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     8461 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     8521 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     8581 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     8641 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     8701 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     8761 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     8821 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     8881 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     8941 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     9001 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     9061 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     9121 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     9181 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga
     9241 tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct
     9301 gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac
     9361 aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct
     9421 gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata
     9481 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     9541 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     9601 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     9661 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gcacgccccc
     9721 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     9781 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     9841 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     9901 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     9961 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
    10021 ctatataagc agcgcgtttt gcctgtactg ggtctctctg gttagaccag atctgagcct
    10081 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
    10141 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
    10201 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
    10261 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
    10321 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
    10381 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
    10441 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
    10501 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
    10561 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
    10621 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
    10681 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
    10741 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
    10801 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
    10861 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
    10921 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
    10981 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
    11041 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
    11101 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
    11161 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
    11221 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
    11281 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
    11341 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
    11401 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
    11461 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
    11521 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
    11581 gatccattcg attagtgaac ggatcggcac tgcgtgcgcc aattctgcag acaaatggca
    11641 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
    11701 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
    11761 attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggtt aattaatacc
    11821 gccatgcatc tgcagagggc cctgcgtatg agtgcaagtg ggttttagga ccaggatgag
    11881 gcggggtggg ggtgcctacc tgacgaccga ccccgaccca ctggacaagc acccaacccc
    11941 cattccccaa attgcgcatc ccctatcaga gagggggagg ggaaacagga tgcggcgagg
    12001 cgcgtgcgca ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc
    12061 gcgccaccgc cg
//
