LOCUS       sequence-16653-e        1316 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 1316)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L2604
FEATURES             Location/Qualifiers
     source          1..1316
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             388..408
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(451..479)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 tctctttaat tttcagagct taaaaatggc tgancncact cacaacgatg gatacgctaa
       61 caacttggaa atgaaataag cttgcatgcc tgcaggcctt ggtcgactct agacactttt
      121 cagctaccta gaattacaat tggatatccc cgcctcccaa tccacccacc cagggaaaaa
      181 gaagggctcg ccgaaaatca aagttatctc caggctcgcg catcccaccg agcggttgac
      241 ttctctccac cacttttcat tttaaccctc ggggtacggg attggccaaa ggacccaaag
      301 gtatgtttcg aatgatacta acataacata gaacattttc aggaggaccc ttcttggagg
      361 gtaccgagct cagaaaaaat gactgctcca aagaagaagc gtaaggtacc ggtagaaaaa
      421 atgagtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
      481 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
      541 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg ggtaagttta
      601 aacatatata tactaactaa ccctgattat ttaaattttc agccaacact tgtcactact
      661 ttctgttatg gtgttcaatg cttctcgaga tacccagatc atatgaaacg gcatgacttt
      721 ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac
      781 gggaactacc agacacgtaa gtttaaacag ttcggtacta actaaccata catatttaaa
      841 ttttcaggtg ctgaagtcna gtttgaaggt gatacccttg ttaatagaat cgagttaaag
      901 gtantngatt ttnaagaaaa tggaaacatt cttggacnca attggaatac anctataact
      961 cacacatgta tacntctggc agacaaccaa agatggaatc nagttgtang ttaaacctga
     1021 ttttacaact aactaatctg atttaatttt caaactncna attagaccac cttganatgg
     1081 agcgttcact agcgacntnt cacaaatacc cnttgggang nccntcnttt acanaancnt
     1141 actgtccnnn atngcccttc aanntccacn aaaaanaccc cngccctctn gattnnaccc
     1201 gngggatacc nggnggggan ntanaaaanc tcngaatccc cggggcgggc ccctaanant
     1261 ttggggaaaa anggcccccc ccaantnacg nttcccaccn aaatattntc cccccc
//
