LOCUS       sequence-166538-         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  induce expression of dipeptide repeat GA in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Jovicic A, Mertens J, Boeynaems S, Bogaert E, Chai N, Yamada SB, 
            Paul JW 3rd, Sun S, Herdy JR, Bieri G, Kramer NJ, Gage FH, Van Den 
            Bosch L, Robberecht W, Gitler AD
  TITLE     Modifiers of C9orf72 dipeptide repeat toxicity connect 
            nucleocytoplasmic transport defects to FTD/ALS.
  JOURNAL   Nat Neurosci. 2015 Sep;18(9):1226-9. doi: 10.1038/nn.4085.
  PUBMED    26308983
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn"; 
            journalName: "Nat Neurosci"; date: "2015-09"; volume: "18"; issue: 
            "9"; pages: "1226-9"
COMMENT     Sequence Label: pAG416-Gal-GA50
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     404..427
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     448..464
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     448..464
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    472..496
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(713..730)
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
ORIGIN
        1 agccgccgag cgggtgacag ccctccgaag gaagactctc ctccgtgcgt cctcgtcttc
       61 accggtcgcg ttcctgaaac gcagatgtgc ctcgcgccgc actgctccga acaataaaga
      121 ttctacaata ctagctttta tggttatgaa gaggaaaaat tggcagtaac ctggccccac
      181 aaaccttcaa atgaacgaat caaattaaca accataggat gataatgcga ttagtttttt
      241 agccttattt ctggggtaat taatcagcga agcgatgatt tttgatctat taacagatat
      301 ataaatgcaa aaactgcata accactttaa ctaatacttt caacattttc ggtttgtatt
      361 acttcttatt caaatgtaat aaaagtatca acaaaaaatt gttaatatac ctctatactt
      421 taacgtcaag gagaaaaaac cccggattct agaactagtg gatcccccat cacaagtttg
      481 tacaaaaaag caggcttcga attcaccatg ggagcaggtg ctggtgcagg agctggagca
      541 ggcgctggcg caggcgctgg agcaggtgct ggtgcaggag ctggcgcagg agctggtgct
      601 ggagctggtg caggagcagg cgctggcgca ggcgcaggtg caggtgcagg tgctggtgca
      661 ggtgctggcg ctggtgctgg tgctggagct ggagctggcg ctggggcagg cgctggtgct
      721 ggtgctggtg c
//
