LOCUS       sequence-166541-         961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  induce expression of dipeptide repeat GA in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Jovicic A, Mertens J, Boeynaems S, Bogaert E, Chai N, Yamada SB, 
            Paul JW 3rd, Sun S, Herdy JR, Bieri G, Kramer NJ, Gage FH, Van Den 
            Bosch L, Robberecht W, Gitler AD
  TITLE     Modifiers of C9orf72 dipeptide repeat toxicity connect 
            nucleocytoplasmic transport defects to FTD/ALS.
  JOURNAL   Nat Neurosci. 2015 Sep;18(9):1226-9. doi: 10.1038/nn.4085.
  PUBMED    26308983
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn"; 
            journalName: "Nat Neurosci"; date: "2015-09"; volume: "18"; issue: 
            "9"; pages: "1226-9"
COMMENT     Sequence Label: pAG416-Gal-GA50
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(4..193)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     205..223
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     241..257
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     242..258
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    291..315
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(326..349)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(368..397)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             478..495
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
     protein_bind    complement(712..736)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(744..760)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(744..760)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(781..804)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 agccttcgag cgtcccaaaa ccttctcaag caaggttttc agtataatgt tacatgcgta
       61 cacgcgtctg tacagaaaaa aaagaaaaat ttgaaatata aataacgttc ttaatactaa
      121 cataactata aaaaaataaa tagggaccta gacttcaggt tgtctaactc cttccttttc
      181 ggttagagcg gatgtggggg gagggcgtga atgtaagcgt gacataacta attacatgac
      241 tcgaggtcga cggtatcgat aagcttgata tcgaattcct gcagcccatc accactttgt
      301 acaagaaagc tgggttagat ctttacttat cgtcgtcatc cttgtaatcc aggatatcat
      361 ttgctgccag atcctcttct gagatgagtt tctgctcagc acctgcacct gcacctgcgc
      421 ctgcgccagc gccagctcca gctccagcac cagcacctgc tccggcacct gctcctgcac
      481 cagcaccagc accagcgcct gccccagcgc cagctccagc tccagcacca gcaccagcgc
      541 cagcacctgc accagcacct gcacctgcac ctgcgcctgc gccagcgcct gctcctgcac
      601 cagctccagc accagctcct gcgccagctc ctgcaccagc acctgctcca gcgcctgcgc
      661 cagcgcctgc tccagctcct gcaccagcac ctgctcccat ggtgaattcg aagcctgctt
      721 ttttgtacaa acttgtgatg ggggatccac tagttctaga atccggggtt ttttctcctt
      781 gacgttaaag tatagaggta tattaacaat tttttgttga tacttttatt acatttgaat
      841 aagaagtaat acaaaccgaa aatgttgaaa gtattagtta aagtggttat gcagtttttg
      901 catttatata tctgttaata gatcaaaaat catcgcttcg ctgattaatt accccagaaa
      961 t
//
