LOCUS       sequence-166543-       12466 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral expression of human VE-cadherin, C-terminally tagged with
            mCitrine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12466)
  AUTHORS   Hayer A, Shao L, Chung M, Joubert LM, Yang HW, Tsai FC, Bisaria A, 
            Betzig E, Meyer T
  TITLE     Engulfed cadherin fingers are polarized junctional structures 
            between collectively migrating endothelial cells.
  JOURNAL   Nat Cell Biol. 2016 Dec;18(12):1311-1323. doi: 10.1038/ncb3438. Epub
            2016 Nov 14.
  PUBMED    27842057
REFERENCE   2  (bases 1 to 12466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb3438"; 
            journalName: "Nat Cell Biol"; date: "2016-12"; volume: "18"; issue: 
            "12"; pages: "1311-1323"
COMMENT     Sequence Label: pLV-CDH5-mCitrine-IRES-puro
FEATURES             Location/Qualifiers
     source          1..12466
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2206..2585
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2588..2863
                     /label=chicken beta-actin promoter
     intron          2864..3872
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3796..3818
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3880..3899
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             complement(5974..5985)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(6299..6323)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      6331..6340
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6337..6339,6340..6342,6343..7056)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6382..6403)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6643..6662)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6990..7011
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(7071..7095)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7213..7762
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7357..7374)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7584..7603
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7765..8364
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7765..7784)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8261..8281
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8388..8976
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8441..8461)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8859..8870)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9184..9817
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9946..9962)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9946..9962)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9959..9981)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9970..9986
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9994..10000,10001..10018,10019..10024))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10039..10060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10177..10194)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10348..10933)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10428..10447)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11104..11895,11896..11964))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11727..11746
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11965..12069)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    12117..12251
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12154..12173)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12208..12227
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgacat tgattattga
     2221 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     2281 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     2341 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     2401 aatgggtgga ctatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     2461 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     2521 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     2581 ccatgggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca
     2641 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg
     2701 gggggggcgc gcgccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggag
     2761 aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg
     2821 gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgttgcc
     2881 ttcgccccgt gccccgctcc gcgccgcctc gcgccgcccg ccccggctct gactgaccgc
     2941 gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt
     3001 ggtttaatga cggctcgttt cttttctgtg gctgcgtgaa agccttaaag ggctccggga
     3061 gggccctttg tgcggggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga
     3121 gcgccgcgtg cggcccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct
     3181 ttgtgcgctc cgcgtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg
     3241 ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg
     3301 gtgtgggcgc ggcggtcggg ctgtaacccc cccctgcacc cccctccccg agttgctgag
     3361 cacggcccgg cttcgggtgc ggggctccgt gcggggcgtg gcgcggggct cgccgtgccg
     3421 ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag
     3481 ggctcggggg aggggcgcgg cggccccgga gcgccggcgg ctgtcgaggc gcggcgagcc
     3541 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa
     3601 tctggcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcgggcgaag
     3661 cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc
     3721 cgtccccttc tccatctcca gcctcggggc tgccgcaggg ggacggctgc cttcgggggg
     3781 gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta
     3841 accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc
     3901 tgtctcatca ttttggcaaa gaattccacc atgcagaggc tcatgatgct cctcgccaca
     3961 tcgggcgcct gcctgggcct gctggcagtg gcagcagtgg cagcagcagg tgctaaccct
     4021 gcccaacggg acacccacag cctgctgccc acccaccggc gccaaaagag agattggatt
     4081 tggaaccaga tgcacattga tgaagagaaa aacacctcac ttccccatca tgtaggcaag
     4141 atcaagtcaa gcgtgagtcg caagaatgcc aagtacctgc tcaaaggaga atatgtgggc
     4201 aaggtcttcc gggtcgatgc agagacagga gacgtgttcg ccattgagag gctggaccgg
     4261 gagaatatct cagagtacca cctcactgct gtcattgtgg acaaggacac tggcgaaaac
     4321 ctggagactc cttccagctt caccatcaaa gttcatgacg tgaacgacaa ctggcctgtg
     4381 ttcacgcatc ggttgttcaa tgcgtccgtg cctgagtcgt cggctgtggg gacctcagtc
     4441 atctctgtga cagcagtgga tgcagacgac cccactgtgg gagaccacgc ctctgtcatg
     4501 taccaaatcc tgaaggggaa agagtatttt gccatcgata attctggacg tattatcaca
     4561 ataacgaaaa gcttggaccg agagaagcag gccaggtatg agatcgtggt ggaagcgcga
     4621 gatgcccagg gcctccgggg ggactcgggc acggccaccg tgctggtcac tctgcaagac
     4681 atcaatgaca acttcccctt cttcacccag accaagtaca catttgtcgt gcctgaagac
     4741 acccgtgtgg gcacctctgt gggctctctg tttgttgagg acccagatga gccccagaac
     4801 cggatgacca agtacagcat cttgcggggc gactaccagg acgctttcac cattgagaca
     4861 aaccccgccc acaacgaggg catcatcaag cccatgaagc ctctggatta tgaatacatc
     4921 cagcaataca gcttcatcgt cgaggccaca gaccccacca tcgacctccg atacatgagc
     4981 cctcccgcgg gaaacagagc ccaggtcatt atcaacatca cagatgtgga cgagcccccc
     5041 attttccagc agcctttcta ccacttccag ctgaaggaaa accagaagaa gcctctgatt
     5101 ggcacagtgc tggccatgga ccctgatgcg gctaggcata gcattggata ctccatccgc
     5161 aggaccagtg acaagggcca gttcttccga gtcacaaaaa agggggacat ttacaatgag
     5221 aaagaactgg acagagaagt ctacccctgg tataacctga ctgtggaggc caaagaactg
     5281 gattccactg gaacccccac aggaaaagaa tccattgtgc aagtccacat tgaagttttg
     5341 gatgagaatg acaatgcccc ggagtttgcc aagccctacc agcccaaagt gtgtgagaac
     5401 gctgtccatg gccagctggt cctgcagatc tccgcaatag acaaggacat aacaccacga
     5461 aacgtgaagt tcaaattcac cttgaatact gagaacaact ttaccctcac ggataatcac
     5521 gataacacgg ccaacatcac agtcaagtat gggcagtttg accgggagca taccaaggtc
     5581 cacttcctac ccgtggtcat ctcagacaat gggatgccaa gtcgcacggg caccagcacg
     5641 ctgaccgtgg ccgtgtgcaa gtgcaacgag cagggcgagt tcaccttctg cgaggatatg
     5701 gccgcccagg tgggcgtgag catccaggca gtggtagcca tcttactctg catcctcacc
     5761 atcacagtga tcaccctgct catcttcctg cggcggcggc tccggaagca ggcccgcgcg
     5821 cacggcaaga gcgtgccgga gatccacgag cagctggtca cctacgacga ggagggcggc
     5881 ggcgagatgg acaccaccag ctacgatgtg tcggtgctca actcggtgcg ccgcggcggg
     5941 gccaagcccc cgcggcccgc gctggacgcc cggccttccc tctatgcgca ggtgcagaag
     6001 ccaccgaggc acgcgcctgg ggcacacgga gggcccgggg agatggcagc catgatcgag
     6061 gtgaagaagg acgaggcgga ccacgacggc gacggccccc cctacgacac gctgcacatc
     6121 tacggctacg agggctccga gtccatagcc gagtccctca gctccctggg caccgactca
     6181 tccgactctg acgtggatta cgacttcctt aacgactggg gacccaggtt taagatgctg
     6241 gctgagctgt acggctcgga cccccgggag gagctgctgt ataagggtgg gcgcgccgac
     6301 ccagctttct tgtacaaagt ggtaccggtc gccaccatgg tgagcaaggg cgaggagctg
     6361 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     6421 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     6481 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc
     6541 ctgatgtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     6601 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     6661 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     6721 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     6781 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     6841 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     6901 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca gtccaagctg
     6961 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     7021 gggatcactc tcggcatgga cgagctgtac aagtaactcg agatatctag acccagcttt
     7081 cttgtacaaa gtggttgata tccagcacag tggcggccgc tctagagtcg acgggccgcg
     7141 gtaacaattg ttaactaact taagctagca acggtttccc tctagcggga tcaattccgc
     7201 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
     7261 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
     7321 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
     7381 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
     7441 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
     7501 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
     7561 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
     7621 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta
     7681 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
     7741 tcctttgaaa aacacgataa taccatgacc gagtacaagc ccacggtgcg cctcgccacc
     7801 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     7861 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     7921 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     7981 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     8041 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     8101 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     8161 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     8221 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     8281 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     8341 atgacccgca agcccggtgc ctgacccggg cggggcgcgt ctggaacaat caacctctgg
     8401 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     8461 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     8521 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     8581 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     8641 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     8701 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     8761 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
     8821 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     8881 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     8941 agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattaattct gcagtcgaga
     9001 cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc
     9061 ctggctagaa gcacaagagg aggaggaggt gggtttttcc agtcacacct caggtacctt
     9121 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaagaggg
     9181 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc
     9241 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc
     9301 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg
     9361 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc
     9421 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc
     9481 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt
     9541 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca
     9601 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc
     9661 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     9721 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     9781 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     9841 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc ttgacattgc
     9901 tagcgtttta ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     9961 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
    10021 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
    10081 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
    10141 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
    10201 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
    10261 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
    10321 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
    10381 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
    10441 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
    10501 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
    10561 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
    10621 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
    10681 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
    10741 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
    10801 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
    10861 caaacaaacc accgctggta gcggtttttt tgtttgcaag cagcagatta cgcgcagaaa
    10921 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
    10981 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
    11041 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
    11101 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
    11161 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
    11221 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
    11281 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
    11341 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
    11401 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
    11461 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
    11521 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
    11581 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
    11641 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    11701 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    11761 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    11821 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    11881 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    11941 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    12001 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    12061 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatcaact
    12121 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
    12181 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
    12241 atgtctggat caactggata actcaagcta accaaaatca tcccaaactt cccaccccat
    12301 accctattac cactgccaat tacctagtgg tttcatttac tctaaacctg tgattcctct
    12361 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt
    12421 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagt
//
