LOCUS       sequence-166544-        3947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for expressing HRP-MDGA2 in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Loh KH, Stawski PS, Draycott AS, Udeshi ND, Lehrman EK, Wilton DK, 
            Svinkina T, Deerinck TJ, Ellisman MH, Stevens B, Carr SA, Ting AY
  TITLE     Proteomic Analysis of Unbounded Cellular Compartments: Synaptic 
            Clefts.
  JOURNAL   Cell. 2016 Aug 25;166(5):1295-1307.e21. doi: 
            10.1016/j.cell.2016.07.041.
  PUBMED    27565350
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.07"; journalName: "Cell"; date: "2016-08-25- 
            25"; volume: "166"; issue: "5"; pages: "1295-1307.e21"
COMMENT     Sequence Label: FSW-HRP-V5-MDGA2
FEATURES             Location/Qualifiers
     source          1..3947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1077..1118
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 gtgcctgaga gcgcagtcta gacgccacca tggatttcct gctgctcggt ctctgtctat
       61 actggctgct gaggaggccc tcgggggtgg tcttgtgtct gctgggggcc tgctttcaga
      121 tgctgcccgc cgctagccag cttactccaa ctttctacga taactcttgt cctaacgtga
      181 gcaacatcgt gcgagatact atcgtgaacg agttgcggtc tgatccgagg atagccgctt
      241 caattttgcg gctgcatttc cacgattgtt ttgtaaacgg ctgtgatgct tccatcctcc
      301 tcgacaatac cacctctttc aggactgaaa aggatgcctt cggtaacgcg aatagtgcta
      361 gagggtttcc cgtgatcgat agaatgaaag ccgctgtgga gagcgcctgc ccccggacag
      421 ttagttgtgc agaccttctg accatagcgg cccagcaatc cgtgacgctg gccggtggcc
      481 ccagctggag ggtccccctg ggaaggaggg actctctgca ggcttttctg gacttggcaa
      541 acgcaaacct gcctgctccc ttcttcaccc ttccccagct gaaggattcc ttcaggaatg
      601 tcggcctgaa ccgaagtagt gatttggtgg ctctgtccgg aggccacaca ttcggcaaga
      661 atcagtgcag gttcatcatg gacagactgt ataacttttc caacaccggc ttgcctgacc
      721 ctaccctgaa cacaacatac ttgcagactc tcagagggct gtgtccgctc aatggcaact
      781 tgtctgcact ggttgacttt gacctgcgca ccccaaccat cttcgacaat aagtattacg
      841 tcaatctgga ggaacagaag ggcctgattc agtccgacca ggagctgttc agttccccaa
      901 atgctaccga cactattcct cttgtgcgca gcttcgctaa tagcacacaa acctttttca
      961 atgctttcgt ggaagctatg gatcggatgg gaaatataac gcccctgacg ggtacgcagg
     1021 gacagataag actgaactgt agggtcgtga acagcaactc tggctcgggc tcgggcggca
     1081 agcccatccc caaccccctg ctgggcctgg acagcaccac cggtcccccg acggttcgga
     1141 ttgtgcactc aggattggcc tgtaatatag aggaggagcg ctactcggaa agggtctata
     1201 ccatccggga aggagaaacg ctagagttga cctgtctggt tactggacat ccacgcccac
     1261 agatcaggtg gaccaaaaca gcaggaagcg cctctgacag attccaagac tcaagtgtct
     1321 tcaatgagac tttgaggatt acaagcattc agagacacca aggaggccga tattactgta
     1381 aagcagagaa tggcttgggg tctccagcaa taaagtcgat ccgagtggat gtgtactact
     1441 tggatgatcc tgtagtaact gttcatcaaa gtataggtga agctaaagaa caattttact
     1501 atgagagaac agtgttcctc cgatgtgtgg ccaattccaa cccccctgtg cgctacagct
     1561 ggagacgtgg ccaagaggtg ttgctacaag gatcagacaa aggagtggag atttacgagc
     1621 ccttttttac ccagggggaa acaaagatct taaagctaaa gaatctccga cctcaggact
     1681 atgctaacta cagctgcatt gcttcagtga ggaacgtgtg taatattcca gacaagatgg
     1741 tgtcttttag actttccaat aaaaccgctt ctccgtcaat taagctcttg gtggatgacc
     1801 ctatagttgt aaatcctgga gaggccataa cgttagtgtg tgttacaaca gggggagagc
     1861 ctatgccctc tctcacctgg gtcaggtcct tcgggactct gcctgaaaag attgttttga
     1921 atggagggac attgaccata cctgccatca cctctgatga tgctgggact tacagctgca
     1981 ttgccaataa taatgtggga aaccctgcaa aaaagtccac caacatcatt gtgagagcat
     2041 taaaaaaagg acgattttgg atcactcctg atccttatca caaagatgac aacatccaga
     2101 ttgggcgtga ggtgaaaata tcttgccaag tagaagccgt tccctctgaa gagttaacat
     2161 tcagttggtt taaaaacggc cgtccattaa gaagttctga gcggatggtt attacacaga
     2221 ctgaccctga tgtgtccccc ggaacaacaa atttggatat cattgattta aaattcacgg
     2281 actttgggac ttacacatgc gtggcctctc tgaagggagg aggaatatct gatatcagta
     2341 ttgacgttaa catttccagc agcacagttc cacccaatct gactgttcca caggaaaaat
     2401 cacctttggt caccagagaa ggggacacaa tagaacttca gtgtcaagta actgggaaac
     2461 caaaaccaat catcctttgg tctagagcag ataaagaagt tgcaatgcct gatgggacaa
     2521 tgcaaatgga gagttatgat gggaccctca ggattgtgaa cgtgtcaagg gaaatgtctg
     2581 ggatgtacag atgccagacc agtcagtaca atggattcaa cgtgaagccc agagaagcct
     2641 tggtgcagct catcgtgcag tatcccccag ctgtggaacc agcattcttg gaaatccgtc
     2701 aaggacaaga cagaagtgtg accatgagct gtagggttct aagagcctat ccgatacgtg
     2761 tgctgactta cgaatggcgt ctgggcaata aattattacg gaccggtcag tttgactctc
     2821 aggagtatac tgagtaccca ctaaagagtc tttccaatga aaactatgga gtttataact
     2881 gcagcatcat taatgaagct ggagcaggga gatgcagctt tcttgtgaca ggaaaagcct
     2941 atgccccaga gttctactat gacacctaca accctgtgtg gcagaaccga caccgagtgt
     3001 attcttacag tctgcagtgg acacaaatga atcccgatgc agtggatcgg attgtcgcat
     3061 accgtttggg tattcggcag gctggacagc aacgttggtg ggaacaggag attaaaataa
     3121 atgggaatat tcaaaaggga gaactaatta catataactt gactgaactt ataaaaccag
     3181 aggcctatga agtccgactg actcctctca ctaaatttgg tgaaggagat tcaacaattc
     3241 gtgtaattaa atatacggca cctgtaaatc ctcatttgag agaatttcac tgtggatttg
     3301 aagatggtaa tatatgctta ttcacccaag atgatacaga taactttgac tggacaaagc
     3361 aaagtactgc aacaagaaat acaaaataca cccctaacac aggtcccaat gcagatcgga
     3421 gtggttccaa agaaggtttt tatatgtaca tcgagacatc gcgacccaga ctagaaggag
     3481 aaaaggctcg acttctcagc cctgtattca gcatagctcc caaaaatcca tatggaccta
     3541 ccaatagtgc atattgtttc agtttcttct atcacatgta tgggcaacat ataggtgttt
     3601 taaatgtata tctacgtttg aaagggcaga cgacaataga gaatcctcta tggtcttcga
     3661 gtgggaacaa aggacaacga tggaatgaag ctcatgttaa tatatatcca attacttcat
     3721 ttcagttaat ttttgaaggc attcggggtc ctgggataga aggtgacatc gccattgacg
     3781 atgtgtcaat tgcagaagga gaatgtgcaa aacaagacct accaactaag aattccgtgg
     3841 atggtgctgt tgggatctta gttcatatat ggctttttcc agttatcatc ctcatctcga
     3901 tcttaagccc tcgaaggtga ggcgcgcccc tgcagggaat tcgatat
//
