LOCUS       sequence-166547-        1275 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for expressing HRP-TM in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Loh KH, Stawski PS, Draycott AS, Udeshi ND, Lehrman EK, Wilton DK, 
            Svinkina T, Deerinck TJ, Ellisman MH, Stevens B, Carr SA, Ting AY
  TITLE     Proteomic Analysis of Unbounded Cellular Compartments: Synaptic 
            Clefts.
  JOURNAL   Cell. 2016 Aug 25;166(5):1295-1307.e21. doi: 
            10.1016/j.cell.2016.07.041.
  PUBMED    27565350
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.07"; journalName: "Cell"; date: "2016-08-25- 
            25"; volume: "166"; issue: "5"; pages: "1295-1307.e21"
COMMENT     Sequence Label: FSW-HRP-TM
FEATURES             Location/Qualifiers
     source          1..1275
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     36..95
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             99..125
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1077..1106
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1110..1256
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
ORIGIN
        1 gtgcctgaga gcgcagtcta gaggatcccg ccaccatgga gacagacaca ctcctgctat
       61 gggtactgct gctctgggtt ccaggttcca ctggtgacta tccatatgat gttccagatt
      121 atgctggggc ccagcagtta acccctacat tctacgacaa tagctgtccc aacgtgtcca
      181 acatcgttcg cgacacaatc gtcaacgagc tcagatccga tcccaggatc gctgcttcaa
      241 tattacgtct gcacttccat gactgcttcg tgaatggttg cgacgctagc atattactgg
      301 acaacaccac cagtttccgc actgaaaagg atgcattcgg gaacgctaac agcgccaggg
      361 gctttccagt gatcgatcgc atgaaggctg ccgttgagtc agcatgccca cgaacagtca
      421 gttgtgcaga cctgctgact atagctgcgc aacagagcgt gactcttgca ggcggaccgt
      481 cctggagagt gccgctcggt cgacgtgact ccctacaggc attcctagat ctggccaacg
      541 ccaacttgcc tgctccattc ttcaccctgc cccagctgaa ggatagcttt agaaacgtgg
      601 gtctgaatcg ctcgagtgac cttgtggctc tgtccggagg acacacattt ggaaagaacc
      661 agtgtaggtt catcatggat aggctctaca atttcagcaa cactgggtta cctgacccca
      721 cgctgaacac tacgtatctc cagacactga gaggcttgtg cccactgaat ggcaacctca
      781 gtgcactagt ggactttgat ctgcggaccc caaccatctt cgataacaag tactatgtga
      841 atctagagga gcagaaaggc ctgatacaga gtgatcaaga actgtttagc agtccaaacg
      901 ccactgacac catcccactg gtgagaagtt ttgctaactc tactcaaacc ttctttaacg
      961 ccttcgtgga agccatggac cgtatgggta acattacccc tctgacgggt acccaaggcc
     1021 agattcgtct gaactgcaga gtggtcaaca gcaactctcc gcggctgcag gtcgacgaac
     1081 aaaaactcat ctcagaagag gatctgaatg ctgtgggcca ggacacgcag gaggtcatcg
     1141 tggtgccaca ctccttgccc tttaaggtgg tggtgatctc agccatcctg gccctggtgg
     1201 tgctcaccat catctccctt atcatcctca tcatgctttg gcagaagaag ccacgttagg
     1261 aattcgatat caagc
//
