LOCUS       sequence-16655-e        1350 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L2678
FEATURES             Location/Qualifiers
     source          1..1350
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             541..561
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(651..673)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ggtcgactct agaggatcaa gagcatttga atcagaatat ggagaacgga gcatgagcat
       61 tttcgaagtt ttttagatgc actagaacaa agcgtgttgg cttcctctga gcccgctttc
      121 cttatatacc cgcattctgc agccttacag aatgttctag aaggtcctag atgcattcgt
      181 ttgaaaatac tcccggtggg tgcaaagaga cgcagacgga aaatgtatct gggtctcttt
      241 attgtgtaca ctacttttcc atgtaccgaa tgtgagtcgc cctccttttg caacaagcag
      301 ctcgaatgtt ctagaaaaag gtggaaaata gtataaatac cgttgaaaat aaataccgaa
      361 caacatttgc tctaattgtg aaattagaaa tcttcaacta taatcatctc actggatccc
      421 cgggattggc caaaggaccc aaaggtatgt ttcgaatgat actaacataa catagaacat
      481 tttcaggagg acccttggct agaggatccc cgggtaccga gctcagaaaa aatgactgct
      541 ccaaagaaga agcgtaaggt accggtgggt gaagaccaga aacagcacct cgaactgagc
      601 cgcgatattg cccagcgttt caacgcgctg tatggcgaga tcgatcccgt cgttttacaa
      661 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
      721 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcga
      781 aggtaagttt aaacagatcc atactaacta acttgttctg acataatttt cagcttgaat
      841 ggcgaatggc gctttgcctg gtttccggca ccagaagcgg tgccggaaag ctggctggag
      901 tgcgatcttc ctgangccga tactgtcgtc gtcccctcaa actggcagat gcacggntac
      961 gatgcgccca tctacaccan cgtgacntat cccattacgg tcaatccgcn gttggttccc
     1021 ccggggatcc gacgggttgg tactcnctcn ctttnatggt gatgaagctg gctacnggaa
     1081 ggcngacccn aattnttttg anggnttnac tcgggnttcc tnggggggca cgggggctgg
     1141 gtcggttccg cccggcaant ttatttantn gancccacna aaaaatngtn ttttttncgg
     1201 gaanccnttg ccccngaatt nacccgggcc ntttttcccc ngaaaanccc ccccgggagg
     1261 gncccctgaa ancccgtttn tggaaanaan attgggggga agggttttcg gnncnntgnn
     1321 aaacaaaaaa aanattntgn nnccnttttg
//
