LOCUS       sequence-166553-         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses BirA*-dCas9-eGFP in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Schmidtmann E, Anton T, Rombaut P, Herzog F, Leonhardt H
  TITLE     Determination of Local Chromatin Composition by CasID.
  JOURNAL   Nucleus. 2016 Sep 27:0.
  PUBMED    27676121
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleus. 
            2016 Sep 27:0."
COMMENT     Sequence Label: pCAG-BirA*-dCas9-eGFP
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(33..53)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      64..73
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(129..149)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ccggtgaaca gctcctcgcc cttgctcacc atgacctttc tcttcttctt tggatgcgat
       61 cgcccatggt ggcgtccacg gcgctgttgc tcgcctgggc tctgctcctc aggccgctct
      121 tgtacaatca ccttcctctt cttcttgggg tcagccctgc tgtctccacc gagctgagag
      181 aggtcgattc ttgtttcata gagccccgta attgactgat gaatcagtgt ggcgtccagg
      241 acctcctttg tagaggtgta ccgctttctg tctatggtgg tgtcgaagta cttgaaggct
      301 gcaggcgcgc ccaagttggt cagagtaaac aagtggataa tgttttctgc ctgctccctg
      361 atgggcttat ccctgtgctt attgtaagca gaaagcacct tatcgaggtt agcgtcggcg
      421 aggatcactc ttttggagaa ttcgcttatt tgctcgatga tctcatcaag gtagtgtttg
      481 tgttgttcca cgaacagctg cttctgctca ttatcttcgg gagacccttt gagcttttca
      541 tagtggctgg ccagatacaa gaaattaacg tatttagagg gcagtgccag ctcgttacct
      601 ttctgcagct cgcccgcact agcgagcatt cgtttccggc cgttttcaag ctcaaagaga
      661 gagtacttgg gaagcttaat gatgaggtct tttttgacct ctttatatcc tttcgcctcg
      721 agaaagtcga tggggttttt ttcgaagctt gatcgctcca tgattgtgat gcccagcagt
      781 tccttgacgc ttttgagttt tttagacttc cctttctcca ctttggccac aaccagtaca
      841 ctgtaagcga ctgtaggaga atcgaatccg ccgtatttct tggggtccca atcttttttg
      901 cgtgcgatca gcttgtcgct gttccttttc gggaggatac tttccttgga gaagcctccg
      961 gtctgtactt cggtctttt
//
