LOCUS       sequence-166555-       10754 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral expression of a stoichiometric F-actin and myosin light 
            chain reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10754)
  AUTHORS   Hayer A, Shao L, Chung M, Joubert LM, Yang HW, Tsai FC, Bisaria A, 
            Betzig E, Meyer T
  TITLE     Engulfed cadherin fingers are polarized junctional structures 
            between collectively migrating endothelial cells.
  JOURNAL   Nat Cell Biol. 2016 Dec;18(12):1311-1323. doi: 10.1038/ncb3438. Epub
            2016 Nov 14.
  PUBMED    27842057
REFERENCE   2  (bases 1 to 10754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb3438"; 
            journalName: "Nat Cell Biol"; date: "2016-12"; volume: "18"; issue: 
            "12"; pages: "1311-1323"
COMMENT     Sequence Label: pLV-Ftractin-mRuby3-p2A-mTurquoise-MLC-IRES-Blast
FEATURES             Location/Qualifiers
     source          1..10754
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2221..2432
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2433..3371
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3328..3348
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3540..4250
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTD
                     IALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAV
                     AKYSNLGGGMDELYK"
     CDS             4260..4316
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(4317..4319,4320..5030)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4359..4380)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4620..4639)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4967..4988
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5672..6221
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5816..5833)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6043..6062
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     misc_feature    6676..7264
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6729..6749)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7147..7158)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7472..8105
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8234..8250)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8234..8250)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8247..8269)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8258..8274
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8282..8288,8289..8306,8307..8312))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8327..8348
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8465..8482)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8636..9221)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8716..8735)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9392..10183,10184..10252))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10015..10034
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10253..10357)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10405..10539
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10442..10461)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10496..10515
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatccacca tgggcatggc gcgaccacgg ggcgcggggc
     3421 cctgcagccc cgggttggag cgggctccgc gccggagcgt cggggagctg cgcctgctct
     3481 tcgaagcgcg ctgcgccgca gtcgccgccg cagcggccgc agggtcggat ccaccggtca
     3541 tggtgtctaa gggcgaagag ctgatcaagg aaaatatgcg tatgaaggtg gtcatggaag
     3601 gttcggtcaa cggccaccaa ttcaaatgca caggtgaagg agaaggcaga ccgtacgagg
     3661 gagtgcaaac catgaggatc aaagtcatcg agggaggacc cctgccattt gcctttgaca
     3721 ttcttgccac gtcgttcatg tatggcagcc gtacctttat caagtacccg gccgacatcc
     3781 ctgatttctt taaacagtcc tttcctgagg gttttacttg ggaaagagtt acgagatacg
     3841 aagatggtgg agtcgtcacc gtcacgcagg acaccagcct tgaggatggc gagctcgtct
     3901 acaacgtcaa ggtcagaggg gtaaactttc cctccaatgg tcccgtgatg cagaagaaga
     3961 ccaagggttg ggagcctaat acagagatga tgtatccagc agatggtggt ctgagaggat
     4021 acactgacat cgcactgaaa gttgatggtg gtggccatct gcactgcaac ttcgtgacaa
     4081 cttacaggtc aaaaaagacc gtcgggaaca tcaagatgcc cggtgtccat gccgttgatc
     4141 accgcctgga aaggatcgag gagagtgaca atgaaaccta cgtagtgcaa agagaagtgg
     4201 cagttgccaa atacagcaac cttggtggtg gcatggacga gctgtacaag ggatccggag
     4261 ccacgaactt ctctctgtta aagcaagcag gagacgtgga agaaaatccc ggtcctgtga
     4321 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     4381 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     4441 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     4501 ccaccctgtc ctggggcgtg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg
     4561 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     4621 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     4681 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     4741 agtacaacta catcagcgac aacgtctata tcaccgccga caagcagaag aacggcatca
     4801 aggccaactt caagatccgc cacaacatcg aggacggcgg cgtgcagctc gccgaccact
     4861 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     4921 gcacccagtc caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     4981 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag ggagccggta
     5041 tgtccagcaa gcgggccaaa gccaagacca ccaagaagcg gccacagcgg gccacatcca
     5101 atgtcttcgc aatgtttgac cagtcccaga tccaggagtt taaggaggct ttcaacatga
     5161 ttgaccagaa ccgtgatggc ttcattgaca aggaggacct gcacgacatg ctggcctcgc
     5221 tggggaagaa ccccacagac gaatacctgg agggcatgat gagcgaggcc ccggggccca
     5281 tcaacttcac catgttcctc accatgtttg gggagaagct gaacggcacg gaccccgagg
     5341 atgtgattcg caacgccttt gcctgcttcg acgaggaagc ctcaggtttc atccatgagg
     5401 accacctccg ggagctgctc accaccatgg gtgaccgctt cacagatgag gaagtggacg
     5461 agatgtaccg ggaggcaccc attgataaga aaggcaactt caactacgtg gagttcaccc
     5521 gcatcctcaa acatggcgcc aaggataaag acgactagga attcctcgag ggcggccgct
     5581 ctagagtcga cgggccgcgg taacaattgt taactaactt aagctagcaa cggtttccct
     5641 ctagcgggat caattccgcc ccccccccct aacgttactg gccgaagccg cttggaataa
     5701 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
     5761 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
     5821 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
     5881 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
     5941 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
     6001 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
     6061 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
     6121 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
     6181 aaccacgggg acgtggtttt cctttgaaaa acacgataat accatggtca tgaaaacatt
     6241 taacatttct caacaagatc tagaattagt agaagtagcg acagagaaga ttacaatgct
     6301 ttatgaggat aataaacatc atgtgggagc ggcaattcgt acgaaaacag gagaaatcat
     6361 ttcggcagta catattgaag cgtatatagg acgagtaact gtttgtgcag aagccattgc
     6421 gattggtagt gcagtttcga atggacaaaa ggattttgac acgattgtag ctgttagaca
     6481 cccttattct gacgaagtag atagaagtat tcgagtggta agtccttgtg gtatgtgtag
     6541 ggagttgatt tcagactatg caccagattg ttttgtgtta atagaaatga atggcaagtt
     6601 agtcaaaact acgattgaag aactcattcc actcaaatat acccgaaatt aacccgggcg
     6661 gggcgcgtct ggaacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     6721 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     6781 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     6841 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     6901 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     6961 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     7021 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc
     7081 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     7141 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     7201 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     7261 ccgcctggaa ttaattctgc agtcgagacc tagaaaaaca tggagcaatc acaagtagca
     7321 atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg
     7381 gtttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat
     7441 cttagccact ttttaaaaga aaagagggga ctggaagggc taattcactc ccaacgaaga
     7501 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac
     7561 tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta
     7621 ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac
     7681 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac
     7741 agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc
     7801 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
     7861 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
     7921 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     7981 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     8041 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     8101 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     8161 atcagagagt gagaggcctt gacattgcta gcgttttacc gtcgacctct agctagagct
     8221 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     8281 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     8341 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     8401 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     8461 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     8521 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     8581 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     8641 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     8701 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     8761 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     8821 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     8881 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     8941 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     9001 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     9061 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     9121 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtttttttg
     9181 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     9241 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     9301 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     9361 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     9421 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     9481 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     9541 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     9601 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     9661 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     9721 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     9781 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     9841 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     9901 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     9961 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
    10021 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    10081 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    10141 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    10201 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    10261 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    10321 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    10381 ctgacgtcga cggatcggga gatcaacttg tttattgcag cttataatgg ttacaaataa
    10441 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
    10501 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac
    10561 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctagtggtt
    10621 tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg
    10681 ttaaatatgt actacaaact tagtagtttt taaagaaatt gtatttgtta aatatgtact
    10741 acaaacttag tagt
//
