LOCUS       sequence-16656-e        1271 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 1271)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L2680
FEATURES             Location/Qualifiers
     source          1..1271
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             544..564
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(607..635)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 tgcngtcgac tctagaggat caagagcatt tgaatcagaa tatggagaac ggagcatgag
       61 cattttcgaa gttttttaga tgcactagaa caaagcgtgt tggcttcctc tgagcccgct
      121 ttccttatat acccgcattc tgcagcctta cagaatgttc tagaaggtcc tagatgcatt
      181 cgtttgaaaa tactcccggt gggtgcaaag agacgcagac ggaaaatgta tctgggtctc
      241 tttattgtgt acactacttt tccatgtacc gaatgtgagt cgccctcctt ttgcaacaag
      301 cagctcgaat gttctagaaa aaggtggaaa atagtataaa taccgttgaa aataaatacc
      361 gaacaacatt tgctctaatt gtgaaattag aaatcttcaa ctataatcat ctcactggat
      421 ccccgggatt ggccaaagga cccaaaggta tgtttcgaat gatactaaca taacatagaa
      481 cattttcagg aggacccttg gctagaggat ccccgggtac cgagctcaga aaaaatgact
      541 gctccaaaga agaagcgtaa ggtaccggta gaaaaaatga gtaaaggaga agaacttttc
      601 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
      661 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc
      721 actactggaa aactacctgt tccatgggta agtttaaaca tatatatact aactaaccct
      781 gattatttaa attttcagcc aacacttgtc actactttct gttatggtgt tcaatgcttc
      841 tcgagatacc cagatcatat gaaacggcat gactttttca agagtgccat gcccgaaggt
      901 tatgtacagg aaagaactat atttttcaaa gatgacggga actacnagac acgtaagttt
      961 aaacagttcg gtactaacta accatacata tttaattttc aggtgctgaa gtcangtttg
     1021 aaggngatac ccttgntaat agaatcnagt taaangnatn gattttaaaa aaanggaaca
     1081 ttctgggaan ccaatnggga aaacaccttn aanncccncn angnttaccc cttgggngaa
     1141 nnacaaaaaa agggaaccca gggtgggggt ttaaacccgn atttncccaa caaccccccc
     1201 ggaattnaat tttccnaact ccnaanttna ncccccctnn gaanggggng gccttcccnn
     1261 ccccaccctt t
//
