LOCUS       sequence-166565-       11642 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drosophila PhiC31 transgenesis vector for cloning rescue transgenes.
            Cloning site is flanked by FRT sequences and contains constitutive 
            GFP reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11642)
  AUTHORS   Zhou Q, Neal SJ, Pignoni F
  TITLE     Mutant Analysis by Rescue Gene Excision: New tools for mosaic 
            studies in Drosophila.
  JOURNAL   Genesis. 2016 Oct 3. doi: 10.1002/dvg.22984.
  PUBMED    27696669
REFERENCE   2  (bases 1 to 11642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genesis. 
            2016 Oct 3. doi: 10.1002/dvg.22984."
COMMENT     Sequence Label: FoRC.attBGm
FEATURES             Location/Qualifiers
     source          1..11642
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(1..792,793..861))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     624..643
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(862..966)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      993..1448
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1080..1099)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1290..1311
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1574..1596
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1588..1605
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1589..1605
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1612..1631
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1612..1630
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            1655..5791
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     complement(1664..1682)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(2134..2205,2607..2880,2955..3609,3671..3986,4207..4338,
                     4409..5023)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    complement(5913..5960)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    complement(6208..6255)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(6966..7682)
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(7009..7029)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     7624..7652
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     protein_bind    complement(9612..9659)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    complement(9907..9954)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    10155..10224
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(10443..10461)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10482..10498)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10482..10498)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10495..10517)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10506..10522
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10530..10536,10537..10554,10555..10560))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10575..10596
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10713..10730)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10884..11472)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10964..10983)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
       61 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
      121 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
      181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
      241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
      301 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
      361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
      421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
      481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
      541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
      601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
      661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
      721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
      781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
      901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
      961 tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc
     1021 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     1081 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     1141 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     1201 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     1261 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     1321 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     1381 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     1441 tacaatttcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     1501 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     1561 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac
     1621 tcactatagg gcgaattggg tacgtaccgg gcccctagta tgtatgtaag ttaataaaac
     1681 ccatttttgc ggaaagtaga taaaaaaaac attttttttt tttactgcac tggatatcat
     1741 tgaacttatc tgatcagttt taaatttact tcgatccaag ggtatttgat gtaccaggtt
     1801 ctttcgatta cctctcactc aaaatgacat tccactcaaa gtcagcgctg tttgcctcct
     1861 tctctgtcca cagaaatatc gccgtctctt tcgccgctgc gtccgctatc tctttcgcca
     1921 ccgtttgtag cgttacgtag cgtcaatgtc cgccttcagt tgcattttgt cagcggtttc
     1981 gtgacgaagc tccaagcggt ttacgccatc aattaaacac aaagtgctgt gccaaaactc
     2041 ctctcgcttc ttatttttgt ttgttttttg agtgattggg gtggtgattg gttttgggtg
     2101 ggtaagcagg ggaaagtgtg aaaaatcccg gcaatgggcc aagaggatca ggagctatta
     2161 attcgcggag gcagcaaaca cccatctgcc gagcatctga acaatgtgag tagtacatgt
     2221 gcatacatct taagttcact tgatctatag gaactgcgat tgcaacatca aattgtctgc
     2281 ggcgtgagaa ctgcgaccca caaaaatccc aaaccgcaat tgcacaaaca aatagtgaca
     2341 cgaaacagat tattctggta gctgttctcg ctatataaga caatttttga gatcatatca
     2401 tgatcaagac atctaaaggc attcattttc gactatattc ttttttacaa aaaatataac
     2461 aaccagatat tttaagctga tcctagatgc acaaaaaata aataaaagta taaacctact
     2521 tcgtaggata cttcggggta ctttttgttc ggggttagat gagcataacg cttgtagttg
     2581 atatttgaga tcccctatca ttgcagggtg acagcggagc ggcttcgcag agctgcatta
     2641 accagggctt cgggcaggcc aaaaactacg gcacgctccg gccacccagt ccgccggagg
     2701 actccggttc agggagcggc caactagccg agaacctcac ctatgcctgg cacaatatgg
     2761 acatctttgg ggcggtcaat cagccgggct ccggatggcg gcagctggtc aaccggacac
     2821 gcggactatt ctgcaacgag cgacacatac cggcgcccag gaaacatttg ctcaagaacg
     2881 gtgagtttct attcgcagtc ggctgatctg tgtgaaatct taataaaggg tccaattacc
     2941 aatttgaaac tcagtttgcg gcgtggccta tccgggcgaa cttttggccg tgatgggcag
     3001 ttccggtgcc ggaaagacga ccctgctgaa tgcccttgcc tttcgatcgc cgcagggcat
     3061 ccaagtatcg ccatccggga tgcgactgct caatggccaa cctgtggacg ccaaggagat
     3121 gcaggccagg tgcgcctatg tccagcagga tgacctcttt atcggctccc taacggccag
     3181 ggaacacctg attttccagg ccatggtgcg gatgccacga catctgacct atcggcagcg
     3241 agtggcccgc gtggatcagg tgatccagga gctttcgctc agcaaatgtc agcacacgat
     3301 catcggtgtg cccggcaggg tgaaaggtct gtccggcgga gaaaggaagc gtctggcatt
     3361 cgcctccgag gcactaaccg atccgccgct tctgatctgc gatgagccca cctccggact
     3421 ggactcattt accgcccaca gcgtcgtcca ggtgctgaag aagctgtcgc agaagggcaa
     3481 gaccgtcatc ctgaccattc atcagccgtc ttccgagctg tttgagctct ttgacaagat
     3541 ccttctgatg gccgagggca gggtagcttt cttgggcact cccagcgaag ccgtcgactt
     3601 cttttcctag tgagttcgat gtgtttatta agggtatcta gcattacatt acatctcaac
     3661 tcctatccag cgtgggtgcc cagtgtccta ccaactacaa tccggcggac ttttacgtac
     3721 aggtgttggc cgttgtgccc ggacgggaga tcgagtcccg tgatcggatc gccaagatat
     3781 gcgacaattt tgctattagc aaagtagccc gggatatgga gcagttgttg gccaccaaaa
     3841 atttggagaa gccactggag cagccggaga atgggtacac ctacaaggcc acctggttca
     3901 tgcagttccg ggcggtcctg tggcgatcct ggctgtcggt gctcaaggaa ccactcctcg
     3961 taaaagtgcg acttattcag acaacggtga gtggttccag tggaaacaaa tgatataacg
     4021 cttacaattc ttggaaacaa attcgctaga ttttagttag aattgcctga ttccacaccc
     4081 ttcttagttt ttttcaatga gatgtatagt ttatagtttt gcagaaaata aataaatttc
     4141 atttaactcg cgaacatgtt gaagatatga atattaatga gatgcgagta acattttaat
     4201 ttgcagatgg ttgccatctt gattggcctc atctttttgg gccaacaact cacgcaagtg
     4261 ggcgtgatga atatcaacgg agccatcttc ctcttcctga ccaacatgac ctttcaaaac
     4321 gtctttgcca cgataaatgt aagtcttgtt tagaatacat ttgcatatta ataatttact
     4381 aactttctaa tgaatcgatt cgatttaggt gttcacctca gagctgccag tttttatgag
     4441 ggaggcccga agtcgacttt atcgctgtga cacatacttt ctgggcaaaa cgattgccga
     4501 attaccgctt tttctcacag tgccactggt cttcacggcg attgcctatc cgatgatcgg
     4561 actgcgggcc ggagtgctgc acttcttcaa ctgcctggcg ctggtcactc tggtggccaa
     4621 tgtgtcaacg tccttcggat atctaatatc ctgcgccagc tcctcgacct cgatggcgct
     4681 gtctgtgggt ccgccggtta tcataccatt cctgctcttt ggcggcttct tcttgaactc
     4741 gggctcggtg ccagtatacc tcaaatggtt gtcgtacctc tcatggttcc gttacgccaa
     4801 cgagggtctg ctgattaacc aatgggcgga cgtggagccg ggcgaaatta gctgcacatc
     4861 gtcgaacacc acgtgcccca gttcgggcaa ggtcatcctg gagacgctta acttctccgc
     4921 cgccgatctg ccgctggact acgtgggtct ggccattctc atcgtgagct tccgggtgct
     4981 cgcatatctg gctctaagac ttcgggcccg acgcaaggag tagccgacat atatccgaaa
     5041 taactgcttg tttttttttt ttaccattat taccatcgtg tttactgttt attgccccct
     5101 caaaaagcta atgtaattat atttgtgcca ataaaaacaa gatatgacct atagaataca
     5161 agtatttccc cttcgaacat ccccacaagt agactttgga tttgtcttct aaccaaaaga
     5221 cttacacacc tgcatacctt acatcaaaaa ctcgtttatc gctacataaa acaccgggat
     5281 atatttttta tatacatact tttcaaatcg cgcgccctct tcataattca cctccaccac
     5341 accacgtttc gtagttgctc tttcgctgtc tcccacccgc tctccgcaac acattcacct
     5401 tttgttcgac gaccttggag cgactgtcgt tagttccgcg cgattcggtt cgctcaaatg
     5461 gttccgagtg gttcatttcg tctcaataga aattagtaat aaatatttgt atgtacaatt
     5521 tatttgctcc aatatatttg tatatatttc cctcacagct atatttattc taatttaata
     5581 ttatgacttt ttaaggtaat tttttgtgac ctgttcggag tgattagcgt tacaatttga
     5641 actgaaagtg acatccagtg tttgttcctt gtgtagatgc atctcaaaaa aatggtgggc
     5701 ataatagtgt tgtttatata tatcaaaaat aacaactata ataataagaa tacatttaat
     5761 ttagaaaatg cttggatttc actggaacta ggctagcgaa ttcgtttgtg gaagcggtat
     5821 tcgcaatatt ttagtagctc gttacagtcc ggtgcgtttt tggttttttg aaagtgcgtc
     5881 ttcagagcgc ttttggtttt caaaagcgct ctgaagttcc tatactttct agagaatagg
     5941 aacttcggaa taggaacttc aaagcgtttc cgaaaacgag cgcttccgaa aatgcaacgc
     6001 gagctgcgca catacagctc actgttcacg tcgcacctat atctgcgtgt tgcctgtata
     6061 tatatataca tgagaagaac ggcatagtgc gtgaattcgt ttgtggaagc ggtattcgca
     6121 atattttagt agctcgttac agtccggtgc gtttttggtt ttttgaaagt gcgtcttcag
     6181 agcgcttttg gttttcaaaa gcgctctgaa gttcctatac tttctagaga ataggaactt
     6241 cggaatagga acttcaaagc gtttccgaaa acgagcgctt ccgaaaatgc aacgcgagct
     6301 gcgcacatac agctcactgt tcacgtcgca cctatatctg cgtgttgcct gtatatatat
     6361 atacatgaga agaacggcat agtgcgtcct gcaggaattc atttaaatgg cgcgccgacg
     6421 tcacgcgtac gtttaaacat atgatcagat cttaattaag gcctaggccg gccgcggccg
     6481 caggtcgaca aaggttaagc aattggctga ccatttaatt ttccacaagt ccaattcaag
     6541 gacaaggaaa acagaataag tacataagac cccttgaaag ttaacgacac ccaacaaaag
     6601 ccggtttact taaatgcaaa tacatttgca gattcgaagc taaaaaggga aaaaaaaacc
     6661 aacaaaaact cgacagctga cttcttttcg tgtatttgta tttattgtat tcgtttattc
     6721 acaaatgaaa tgcaaaattc tcccgagtgt tgcgcctgtt cttgttacaa aaagtataaa
     6781 ctcaaaggga gcaatgaatc ctccagttgt ttagcctaat tatacacact aaaattttgt
     6841 ttttttttta ctagaggatc gtacggatct gggggatcga tcccccctgc ccggttatta
     6901 ttatttttga caccagacca actggtaatg gtagcgaccg gcgctcagct ggaattctac
     6961 gaatgctatt tgtatagttc atccatgcca tgtgtaatcc cagcagctgt tacaaactca
     7021 agaaggacca tgtggtctct cttttcgttg ggatctttcg aaagggcaga ttgtgtggac
     7081 aggtaatggt tgtctggtaa aaggacaggg ccatcgccaa ttggagtatt ttgttgataa
     7141 tggtctgcta gttgaacgct tccatcttca atgttgtgtc taattttgaa gttaactttg
     7201 attccattct tttgtttgtc tgccatgatg tatacattgt gtgagttata gttgtattcc
     7261 aatttgtgtc caagaatgtt tccatcttct ttaaaatcaa taccttttaa ctcgattcta
     7321 ttaacaaggg tatcaccttc aaacttgact tcagcacgtg tcttgtagtt cccgtcatct
     7381 ttgaaaaata tagttctttc ctgtacataa ccttcgggca tggcactctt gaaaaagtca
     7441 tgccgtttca tatgatctgg gtatcttgaa aagcattgaa caccataaga gaaagtagtg
     7501 acaagtgttg gccatggaac aggtagtttt ccagtagtgc aaataaattt aagggtaagt
     7561 tttccgtatg ttgcatcacc ttcaccctct ccactgacag aaaatttgtg cccattaaca
     7621 tcaccatcta attcaacaag aattgggaca actccagtga aaagttcttc tcctttactc
     7681 attttttcta ccggtacccg gggatccccg atctgcattt tggattattc tgcgggcaga
     7741 aaatagagat gtggaaaatt agtacgaaat caaatgagtt tcgttgaaat tacaaaacta
     7801 ttgaaactaa cttcctggct ggggaataaa aatgggaaac ttatttatcg acgccaactt
     7861 tgttgagaaa cccctattaa ccctctacga atattggaac aaaggaaagc gaagaaacag
     7921 ggaacaaagg gtagttgaga aacctttgtt ccgttgcttc gtcatcgttt tcgtgttgcg
     7981 agtgtgtgca tgtatatata cacagctgaa atgcatgcat acacattatt ttgtgtgtat
     8041 atggtgacgt cacaactaca agcaataaga aattttcaga cgtggctttg tttaagcaac
     8101 ctactctatt tcagctaaaa ataagtggat ttcgttggta aaatacttca attaactaaa
     8161 caactaacta ataacatgca cacaaatgct cgagtgcgtt cgtgatttct cgaattttca
     8221 aatgcgtcac tgcgaatttc acaatttgcc aataaatctt ggcgaaaatc aacacgcaag
     8281 ttttatttat agatttgttt gcgttttgat gccaattcat tgggaaaaca agatgcgtgg
     8341 ctgccaattt cttattttgt aattacgtag agcgttgaat aaaaaaaaaa tggccgaaca
     8401 aagaccttga aatgcagttt ttcttgaaat tactcaacgt cttgttgctc ttattactaa
     8461 ttggtaacag cgagttaaaa acttacgttt cttgtgactt tcgagaatgt tcttttaatt
     8521 gtactttaat caccaacaat taagtataaa tttttcgctg attgcgcttt actttctact
     8581 tgtacttgct gctgcaaatg tcaattggtt ttgaaggcga ccgttcgcga acgctgttta
     8641 tatacctttc ggtgtccgtt gaaaatcact aaaaaatacc gtagtgttcg taacacttta
     8701 gtacagagaa aaaaaattgt gccgaaatgt ttttgatacg tacgaatacc ttgtattaaa
     8761 attttttatg atttctgtgt atcacttttt ttttgtgttt ttcgtttaaa ctcaccacag
     8821 tacaaaacaa taaaatattt ttaagacaat ttcaaattga gacctttctc gtactgactt
     8881 gaccggctga atgaggattt ctacctagac gacctacttc ttaccatgac attgaatgca
     8941 atgccacctt tgatctaaac ttacaaaagt ccaaggcttg ttaggattgg tgttttggaa
     9001 tttaagaccc ttgttagttt gcttttgaaa tagcactgtc ttctctaccg gctataattt
     9061 tgaaactcgc agcttgactg gaaatttaaa aagtaattct gtgtaggtaa agggtgtttt
     9121 aaaagtgtga tgtgttgagc gttgcggcaa cgactgctat ttatgtatat attttcaaaa
     9181 cttattgttt ttgaagtgtt ttaaatggag ctatctggca acgctgcgca taatcttaca
     9241 caagcttttc ttaatccatt tttaagtgaa atttgtttat actctttcgg caaataattg
     9301 ttaaatcgct ttaagtgggc ttacatctgg ataagtaatg aaaacctgca tattataata
     9361 ttaaaacata taatccactg tgctttcccc gtgtgtggcc atatacctaa aaaagtttat
     9421 tttcgcagag ccccgcacgg tcacactacg gttcggcgat tttcgatttt ggacagtggg
     9481 gtaccggcgc gccggcgaat tcgtttgtgg aagcggtatt cgcaatattt tagtagctcg
     9541 ttacagtccg gtgcgttttt ggttttttga aagtgcgtct tcagagcgct tttggttttc
     9601 aaaagcgctc tgaagttcct atactttcta gagaatagga acttcggaat aggaacttca
     9661 aagcgtttcc gaaaacgagc gcttccgaaa atgcaacgcg agctgcgcac atacagctca
     9721 ctgttcacgt cgcacctata tctgcgtgtt gcctgtatat atatatacat gagaagaacg
     9781 gcatagtgcg tgaattcgtt tgtggaagcg gtattcgcaa tattttagta gctcgttaca
     9841 gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga gcgcttttgg ttttcaaaag
     9901 cgctctgaag ttcctatact ttctagagaa taggaacttc ggaataggaa cttcaaagcg
     9961 tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg cgcacataca gctcactgtt
    10021 cacgtcgcac ctatatctgc gtgttgcctg tatatatata tacatgagaa gaacggcata
    10081 gtgcgtgaat tcgcccggcc gcggctcgag ggtacctcta gagatccact agtgtcgacg
    10141 atgtaggtca cggtctcgaa gccgcggtgc gggtgccagg gcgtgccctt gggctccccg
    10201 ggcgcgtact ccacctcacc catctggtcc atcatgatga acgggtcgag gtggcggtag
    10261 ttgatcccgg cgaacgcgcg gcgcaccggg aagccctcgc cctcgaaacc gctgggcgcg
    10321 gtggtcacgg tgagcacggg acgtgcgacg gcgtcggcgg gtgcggatac gcggggcagc
    10381 gtcagcgggt tctcgacggt cacggcgggc atgtcgacac tagttctagc cagcttttgt
    10441 tccctttagt gagggttaat ttcgagcttg gcgtaatcat ggtcatagct gtttcctgtg
    10501 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
    10561 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
    10621 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
    10681 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
    10741 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
    10801 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
    10861 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
    10921 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
    10981 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
    11041 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
    11101 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
    11161 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    11221 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    11281 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
    11341 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    11401 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    11461 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
    11521 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
    11581 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
    11641 ag
//
