LOCUS       sequence-16657-e        1347 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 1347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L2681
FEATURES             Location/Qualifiers
     source          1..1347
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             548..568
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(611..639)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gngctgcggt cgactctaga ggatcaagag catttgaatc agaatatgga gaacggagca
       61 tgancntttt cgaagttttt tagatgcact agaacaaagc gtgttgnctt cctctgagcc
      121 cgctttcctt atatacccgc attctgcagc cttacagaat gttctagaag gtcctagatg
      181 cattcgtttg aaaatactcc cggtgggtgc aaagagacgc agacggaaaa tgtatctggg
      241 tctctttatt gtgtacacta cttttccatg taccgaatgt gagtcgccct ccttttgcaa
      301 caagcagctc gaatgttcta gaaaaaggtg gaaaatagta taaataccgt tgaaaataaa
      361 taccgaacaa catttgctct aattgtgaaa ttagaaatct tcaactataa tcatctcact
      421 ggatccccgg gattggccaa aggacccaaa ggtatgtttc gaatgatact aacataacat
      481 agaacatttt caggaggacc cttggctaga ggatccccgg gtaccgagct cagaaaaaat
      541 gactgctcca aagaagaagc gtaaggtacc ggtagaaaaa atgagtaaag gagaagaact
      601 tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt
      661 ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat
      721 ttgcactact ggaaaactac ctgttccatg ggtaagttta aacatatata tactaactaa
      781 ccctgattat ttaaattttc agccaacact tgtcactact ttctgttatg gtgttcaatg
      841 cttctcgaga tacccagatc atatgaaacg gcatgacttt ttcaagagtg ccatgcccga
      901 aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtag
      961 tttaaacagt tcggtactaa ctaaccatac atatttaatt ttcaggtgct gaagtcangt
     1021 ttgaaggtga tacccttgtt aatagaatcg agttaaangn atggatttta aagaaaaggg
     1081 aacattcttg ggacncaatt ggaatacacc tnnactccnc natgnttacc tctgggcgan
     1141 aacnaaanaa ggaatcaagt ggaagtttaa ccngatttcc aaccacaacc ggattaattn
     1201 ccanctcnaa taaccncntg gaaaggannt ncctgcnacn tttcnnaatn ccttgggngg
     1261 ccgtcttnca aanatncggn ccnattgcnt taaanccaaa aaaaccngnc ttnnntnacc
     1321 ggggaaccng gggnaanaaa ngcgggg
//
