LOCUS       sequence-166570-         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Drosophila P-element transgenesis vector for cloning rescue 
            transgenes. Cloning site is flanked by FRT sequences and contains 
            constitutive GFP reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Zhou Q, Neal SJ, Pignoni F
  TITLE     Mutant Analysis by Rescue Gene Excision: New tools for mosaic 
            studies in Drosophila.
  JOURNAL   Genesis. 2016 Oct 3. doi: 10.1002/dvg.22984.
  PUBMED    27696669
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genesis. 
            2016 Oct 3. doi: 10.1002/dvg.22984."
COMMENT     Sequence Label: FoRC.PGa
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     194..212
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    complement(386..433)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    complement(681..728)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     878..895
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     879..895
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ttctgtggac agagaaggag gcaaacagcg ctgactttga gtggaatgtc attttgagtg
       61 agaggtaatc gaaagaacct ggtacatcaa atacccttgg atcgaagtaa atttaaaact
      121 gatcagataa gttcaatgat atccagtgca gtaaaaaaaa aaaatgtttt ttttatctac
      181 tttccgcaaa aatgggtttt attaacttac atacatacta gaattcgtta acagatctgc
      241 ggccgcgaat tcactagtga ttaagctagc gaattcgttt gtggaagcgg tattcgcaat
      301 attttagtag ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc gtcttcagag
      361 cgcttttggt tttcaaaagc gctctgaagt tcctatactt tctagagaat aggaacttcg
      421 gaataggaac ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa cgcgagctgc
      481 gcacatacag ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt atatatatat
      541 acatgagaag aacggcatag tgcgtgaatt cgtttgtgga agcggtattc gcaatatttt
      601 agtagctcgt tacagtccgg tgcgtttttg gttttttgaa agtgcgtctt cagagcgctt
      661 ttggttttca aaagcgctct gaagttccta tactttctag agaataggaa cttcggaata
      721 ggaacttcaa agcgtttccg aaaacgagcg cttccgaaaa tgcaacgcga gctgcgcaca
      781 tacagctcac tgttcacgtc gcacctatat ctgcgtgttg cctgtatata tatatacatg
      841 agaagaacgg catagtgcgt cctgcaggag gccggcctgt aaaacgacgg ccagtcttaa
      901 gctcgggccc caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaa
//
