LOCUS       sequence-166579-       14575 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drosophila P-element transgenesis vector for cloning rescue 
            transgenes. Cloning site is flanked by FRT sequences and contains 
            constitutive GFP reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14575)
  AUTHORS   Zhou Q, Neal SJ, Pignoni F
  TITLE     Mutant Analysis by Rescue Gene Excision: New tools for mosaic 
            studies in Drosophila.
  JOURNAL   Genesis. 2016 Oct 3. doi: 10.1002/dvg.22984.
  PUBMED    27696669
REFERENCE   2  (bases 1 to 14575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genesis. 
            2016 Oct 3. doi: 10.1002/dvg.22984."
COMMENT     Sequence Label: FoRC.PGa
FEATURES             Location/Qualifiers
     source          1..14575
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(820..1408)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(900..919)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1579..2370,2371..2439))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2202..2221
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2440..2544)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2612..2630
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2668..2690)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2790..2809
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(3346..3376,3377..3578)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    3765..3812
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    4060..4107
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6044..6760
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(6074..6102)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     6697..6717
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     7274..7290
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7274..7290
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7295..7314
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7295..7313
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    7332..7563
                     /gene="mutant version of attP"
                     /label=attP2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        7708..7810
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             7811..8494
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     complement(7877..7896)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             8814..9119
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8998..9017
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(9515..9746)
                     /gene="mutant version of attP"
                     /label=attP1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(9763..9780)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9763..9779)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9930..9977
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    10225..10272
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     gene            10437..14564
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     complement(10446..10464)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     misc_feature    join(14575,1..554,555..585)
                     /label=P element 5' end
ORIGIN
        1 ctgctgctct aaacgacgca tttcgtactc caaagtacga attttttccc tcaagctctt
       61 attttcatta aacaatgaac aggacctaac gcacagtcac gttattgttt acataaatga
      121 ttttttttac tattcaaact tactctgttt gtgtactccc actggtatag ccttctttta
      181 tcttttctgg ttcaggctct atcactttac taggtacggc atctgcgttg agtcgcctcc
      241 ttttaaatgt ctgacctttt gcaggtgcag ccttccactg cgaatcttta aagtgggtat
      301 cacaaatttg ggagttttca ccaaggctgc acccaaggct ctgctcccac aattttctct
      361 taatagcaca cttcggcacg tgaattaatt ttactccagt cacagctttg cagcaaaatt
      421 tgcaatattt catttttttt tattccacgt aagggttaat gttttcaaaa aaaaattcgt
      481 ccgcacacaa cctttcctct caacaagcaa acgtgcactg aatttaagtg tatacttcgg
      541 taagcttcgg ctatcgacgg gaccacctta tgttatttca tcatgggcca gacccacgta
      601 gtccagcggc agatcggcgg cggagaagtt aagcgtctcc aggatgacct tgcccgaact
      661 ggggcacgtg gtgttcgacg atgtgcagct aatttcgccc ggctccacgt ccgcccattg
      721 gttaatcagc agaccctcgt tggcgtaacg gaaccatgag aggtacgaca accatttgag
      781 gtatactggc accgagcccg agttcaagaa gaaggcgttt ttccataggc tccgcccccc
      841 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
      901 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
      961 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc
     1021 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     1081 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     1141 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     1201 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     1261 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     1321 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     1381 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     1441 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     1501 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     1561 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     1621 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     1681 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     1741 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     1801 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     1861 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     1921 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     1981 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     2041 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     2101 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     2161 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     2221 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     2281 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     2341 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     2401 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     2461 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     2521 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     2581 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     2641 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     2701 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     2761 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     2821 ccatatgcgg tgtgaaatac cgcaccgaat cgcgcggaac taacgacagt cgctccaagg
     2881 tcgtcgaaca aaaggtgaat gtgttgcgga gagcgggtgg gagacagcga aagagcaact
     2941 acgaaacgtg gtgtggtgga ggtgaattat gaagagggcg cgcgatttga aaagtatgta
     3001 tataaaaaat atatcccggt gttttatgta gcgataaacg agtttttgat gtaaggtatg
     3061 caggtgtgta agtcttttgg ttagaagaca aatccaaagt ctacttgtgg ggatgttcga
     3121 aggggaaata cttgtattct ataggtcata tcttgttttt attggcacaa atataattac
     3181 attagctttt tgagggggca ataaacagta aacacgatgg taataatggt aaaaaaaaaa
     3241 acaagcagtt atttcggata tatgtcggct actccttgcg tcgggcccga agtcttagag
     3301 ccagatatgc gagcacccgg aagctcacga tgagaatggc cagaccatga tgaaataaca
     3361 taaggtggtc ccgtcggcaa gagacatcca cttaacgtat gcttgcaata agtgcgagtg
     3421 aaaggaatag tattctgagt gtcgtattga gtctgagtga gacagcgata tgattgttga
     3481 ttaaccctta gcatgtccgt ggggtttgaa ttaactcata atattaatta gacgaaatta
     3541 tttttaaagt tttattttta ataatttgcg agtacgcaaa gcttggctgc aggtcgactc
     3601 tagaggatct ggtctagagc ccgggcgaat tcacgcacta tgccgttctt ctcatgtata
     3661 tatatataca ggcaacacgc agatataggt gcgacgtgaa cagtgagctg tatgtgcgca
     3721 gctcgcgttg cattttcgga agcgctcgtt ttcggaaacg ctttgaagtt cctattccga
     3781 agttcctatt ctctagaaag tataggaact tcagagcgct tttgaaaacc aaaagcgctc
     3841 tgaagacgca ctttcaaaaa accaaaaacg caccggactg taacgagcta ctaaaatatt
     3901 gcgaataccg cttccacaaa cgaattcacg cactatgccg ttcttctcat gtatatatat
     3961 atacaggcaa cacgcagata taggtgcgac gtgaacagtg agctgtatgt gcgcagctcg
     4021 cgttgcattt tcggaagcgc tcgttttcgg aaacgctttg aagttcctat tccgaagttc
     4081 ctattctcta gaaagtatag gaacttcaga gcgcttttga aaaccaaaag cgctctgaag
     4141 acgcactttc aaaaaaccaa aaacgcaccg gactgtaacg agctactaaa atattgcgaa
     4201 taccgcttcc acaaacgaat tcgccggcgc gccggtaccc cactgtccaa aatcgaaaat
     4261 cgccgaaccg tagtgtgacc gtgcggggct ctgcgaaaat aaactttttt aggtatatgg
     4321 ccacacacgg ggaaagcaca gtggattata tgttttaata ttataatatg caggttttca
     4381 ttacttatcc agatgtaagc ccacttaaag cgatttaaca attatttgcc gaaagagtat
     4441 aaacaaattt cacttaaaaa tggattaaga aaagcttgtg taagattatg cgcagcgttg
     4501 ccagatagct ccatttaaaa cacttcaaaa acaataagtt ttgaaaatat atacataaat
     4561 agcagtcgtt gccgcaacgc tcaacacatc acacttttaa aacacccttt acctacacag
     4621 aattactttt taaatttcca gtcaagctgc gagtttcaaa attatagccg gtagagaaga
     4681 cagtgctatt tcaaaagcaa actaacaagg gtcttaaatt ccaaaacacc aatcctaaca
     4741 agccttggac ttttgtaagt ttagatcaaa ggtggcattg cattcaatgt catggtaaga
     4801 agtaggtcgt ctaggtagaa atcctcattc agccggtcaa gtcagtacga gaaaggtctc
     4861 aatttgaaat tgtcttaaaa atattttatt gttttgtact gtggtgagtt taaacgaaaa
     4921 acacaaaaaa aaagtgatac acagaaatca taaaaaattt taatacaagg tattcgtacg
     4981 tatcaaaaac atttcggcac aatttttttt ctctgtacta aagtgttacg aacactacgg
     5041 tattttttag tgattttcaa cggacaccga aggtatataa acagcgttcg cgaacggtcg
     5101 ccttcaaaac caattgacat ttgcagcagc aagtacaagt agaaagtaaa gcgcaatcag
     5161 cgaaaaattt atacttaatt gttggtgatt aaagtacaat taaaagaaca ttctcgaaag
     5221 tcacaagaaa cgtaagtttt taactcgctg ttaccaatta gtaataagag caacaagacg
     5281 ttgagtaatt tcaagaaaaa ctgaatttca aggtctttgt tcggccattt tttttttatt
     5341 caacgctcta cgtaattaca aaataagaaa ttggcagcca cgcatcttgt tttcccaatg
     5401 aattggcatc aaaacgcaaa caaatctata aataaaactt gcgtgttgat tttcgccaag
     5461 atttattggc aaattgtgaa attcgcagtg acgcatttga aaattcgaga aatcacgaac
     5521 gcactcgagc atttgtgtgc atgttattag ttagttgttt agttaattga agtattttac
     5581 caacgaaatc cacttatttt tagctgaaat agagtaggtt gcttaaacaa agccacgtct
     5641 gaaaatttct tattgcttgt agttgtgacg tcaccatata cacacaaaat aatgtgtatg
     5701 catgcatttc agctgtgtat atatacatgc acacactcgc aacacgaaaa cgatgacgaa
     5761 gcaacggaac aaaggtttct caactaccct ttgttccctg tttcttcgct ttcctttgtt
     5821 ccaatattcg tagagggtta ataggggttt ctcaacaaag ttggcgtcga taaataagtt
     5881 tcccattttt attccccagc caggaagtta gtttcaatag ttttgtaatt tcaacgaaac
     5941 tcatttgatt tcgtactaat tttccacatc tctattttct gcccgcagaa taatccaaag
     6001 cgatcgcatg cagatcgggg atccccgggt accggtagaa aaaatgagta aaggagaaga
     6061 acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa
     6121 attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt
     6181 tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta ctttctctta
     6241 tggtgttcaa tgcttttcaa gatacccaga tcatatgaaa cggcatgact ttttcaagag
     6301 tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta
     6361 caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa
     6421 aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa
     6481 ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa
     6541 aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac
     6601 tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc
     6661 cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc
     6721 tgctgggatt acacatggca tggatgaact atacaaatag cattcgtaga attccagctg
     6781 agcgatcgcg cgccggtcgc taccattacc agttggtctg gtgtcaaaaa taataataac
     6841 cgggcagggg ggatcgatcc cccagatccg tacgatcctc tagtaaaaaa aaaacaaaat
     6901 tttagtgtgt ataattaggc taaacaactg gaggattcat tgctcccttt gagtttatac
     6961 tttttgtaac aagaacaggc gcaacactcg ggagaatttt gcatttcatt tgtgaataaa
     7021 cgaatacaat aaatacaaat acacgaaaag aagtcagctg tcgagttttt gttggttttt
     7081 ttttcccttt ttagcttcga atctgcaaat gtatttgcat ttaagtaaac cggcttttgt
     7141 tgggtgtcgt taactttcaa ggggtcttat gtacttattc tgttttcctt gtccttgaat
     7201 tggacttgtg gaaaattaaa tggtcagcca attgcttaac ctttgtcgac ctgcggccgc
     7261 ggtggccggc cgccaggaaa cagctatgac catgtaatac gactcactat aggggatatc
     7321 agctggatgg caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat
     7381 tgataagcaa tgctttctta taatgccaac tttgtacaag aaagctgaac gagaaacgta
     7441 aaatgatata aatatcaata tattaaatta gactttgcat aaaaaacaga ctacataata
     7501 ctgtaaaaca caacatatcc agtcactatg aatcaactac ttagatggta ttagtgacct
     7561 gtagtcgact aagttggcag catcacccga cgcactttgc gccgaataaa tacctgtgac
     7621 ggaagatcac ttcgcagaat aaataaatcc tggtgtccct gttgataccg ggaagccctg
     7681 ggccaacttt tggcgaaaat gagacgttga tcggcacgta agaggttcca actttcacca
     7741 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa
     7801 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
     7861 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
     7921 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
     7981 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat
     8041 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
     8101 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
     8161 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
     8221 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga
     8281 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta
     8341 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     8401 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg
     8461 ggggcgtaaa cgccgcgtgg atccggctta ctaaaagcca gataacagta tgcgtatttg
     8521 cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa
     8581 agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc
     8641 tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc
     8701 ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc
     8761 ccggtttatt gaaatgaacg gctcttttgc tgacgagaac aggggctggt gaaatgcagt
     8821 ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg
     8881 atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt
     8941 cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca
     9001 tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc
     9061 tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa
     9121 tgtcaggctc ccttatacac agccagtctg caggtcgata cagtagaaat tacagaaact
     9181 ttatcacgtt tagtaagtat agaggctgaa aatccagatg aagccgaacg acttgtaaga
     9241 gaaaagtata agagttgtga aattgttctt gatgcagatg attttcagga ctatgacact
     9301 agcgtatatg aataggtaga tgtttttatt ttgtcacaca aaaaagaggc tcgcacctct
     9361 ttttcttatt tctttttatg atttaatacg gcattgagga caatagcgag taggctggat
     9421 acgacgattc cgtttgagaa gaacatttgg aaggctgtcg gtcgactaag ttggcagcat
     9481 cacccgaaga acatttggaa ggctgtcggt cgactacagg tcactaatac catctaagta
     9541 gttgattcat agtgactgga tatgttgtgt tttacagtat tatgtagtct gttttttatg
     9601 caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt cagctttttt
     9661 gtacaaagtt ggcattataa aaaagcattg ctcatcaatt tgttgcaacg aacaggtcac
     9721 tatcagtcaa aataaaatca ttatttgggg cccgagctta agactggccg tcgttttaca
     9781 ggccggcctc ctgcaggacg cactatgccg ttcttctcat gtatatatat atacaggcaa
     9841 cacgcagata taggtgcgac gtgaacagtg agctgtatgt gcgcagctcg cgttgcattt
     9901 tcggaagcgc tcgttttcgg aaacgctttg aagttcctat tccgaagttc ctattctcta
     9961 gaaagtatag gaacttcaga gcgcttttga aaaccaaaag cgctctgaag acgcactttc
    10021 aaaaaaccaa aaacgcaccg gactgtaacg agctactaaa atattgcgaa taccgcttcc
    10081 acaaacgaat tcacgcacta tgccgttctt ctcatgtata tatatataca ggcaacacgc
    10141 agatataggt gcgacgtgaa cagtgagctg tatgtgcgca gctcgcgttg cattttcgga
    10201 agcgctcgtt ttcggaaacg ctttgaagtt cctattccga agttcctatt ctctagaaag
    10261 tataggaact tcagagcgct tttgaaaacc aaaagcgctc tgaagacgca ctttcaaaaa
    10321 accaaaaacg caccggactg taacgagcta ctaaaatatt gcgaataccg cttccacaaa
    10381 cgaattcgct agcttaatca ctagtgaatt cgcggccgca gatctgttaa cgaattctag
    10441 tatgtatgta agttaataaa acccattttt gcggaaagta gataaaaaaa acattttttt
    10501 tttttactgc actggatatc attgaactta tctgatcagt tttaaattta cttcgatcca
    10561 agggtatttg atgtaccagg ttctttcgat tacctctcac tcaaaatgac attccactca
    10621 aagtcagcgc tgtttgcctc cttctctgtc cacagaaata tcgccgtctc tttcgccgct
    10681 gcgtccgcta tctctttcgc caccgtttgt agcgttacct agcgtcaatg tccgccttca
    10741 gttgcacttt gtcagcggtt tcgtgacgaa gctccaagcg gtttacgcca tcaattaaac
    10801 acaaagtgct gtgccaaaac tcctctcgct tcttattttt gtttgttttt tgagtgattg
    10861 gggtggtgat tggttttggg tgggtaagca ggggaaagtg tgaaaaatcc cggcaatggg
    10921 ccaagaggat caggagctat taattcgcgg aggcagcaaa cacccatctg ccgagcatct
    10981 gaacaatgtg agtagtacat gtgcatacat cttaagttca cttgatctat aggaactgcg
    11041 attgcaacat caaattgtct gcggcgtgag aactgcgacc cacaaaaatc ccaaaccgca
    11101 atcgcacaaa caaatagtga cacgaaacag attattctgg tagctgtgct cgctatataa
    11161 gacaattttt aagatcatat catgatcaag acatctaaag gcattcattt tcgactacat
    11221 tcttttttac aaaaaatata acaaccagat attttaagct gatcctagat gcacaaaaaa
    11281 taaataaaag tataaaccta cttcgtagga tacttcgttt tgttcggggt tagatgagca
    11341 taacgcttgt agttgatatt tgagatcccc tatcattgca gggtgacagc ggacgcttcg
    11401 cagagctgca ttaaccaggg cttcgggcag gccaaaaact acggcacgct cctgccaccc
    11461 agtccgccgg aggactccgg ttcagggagc ggccaactag ccgagaacct cacctatgcc
    11521 tggcacaata tggacatctt tggggcggtc aatcagccgg gctccggatg gcggcagctg
    11581 gtcaaccgga cacgcggact attctgcaac gagcgacaca taccggcgcc caggaaacat
    11641 ttgctcaaga acggtgagtt tctattcgca gtcggctgat ctgtgtgaaa tcttaataaa
    11701 gggtccaatt accaatttga aactcagttt gcggcgtggc ctatccgggc gaacttttgg
    11761 ccgtgatggg cagttccggt gccggaaaga cgaccctgct gaatgccctt gcctttcgat
    11821 cgccgcaggg catccaagta tcgccatccg ggatgcgact gctcaatggc caacctgtgg
    11881 acgccaagga gatgcaggcc aggtgcgcct atgtccagca ggatgacctc tttatcggct
    11941 ccctaacggc cagggaacac ctgattttcc aggccatggt gcggatgcca cgacatctga
    12001 cctatcggca gcgagtggcc cgcgtggatc aggtgatcca ggagctttcg ctcagcaaat
    12061 gtcagcacac gatcatcggt gtgcccggca gggtgaaagg tctgtccggc ggagaaagga
    12121 agcgtctggc attcgcctcc gaggcactaa ccgatccgcc gcttctgatc tgcgatgagc
    12181 ccacctccgg actggactca tttaccgccc acagcgtcgt ccaggtgctg aagaagctgt
    12241 cgcagaaggg caagaccgtc atcctgacca ttcatcagcc gtcttccgag ctgtttgagc
    12301 tctttgacaa gatccttctg atggccgagg gcagggtagc tttcttgggc actcccagcg
    12361 aagccgtcga cttcttttcc tagtgagttc gatgtgttta ttaagggtat ctagcattac
    12421 attacatctc aactcctatc cagcgtgggt gcccagtgtc ctaccaacta caatccggcg
    12481 gacttttacg tacaggtgtt ggccgttgtg cccggacggg agatcgagtc ccgtgatcgg
    12541 atcgccaaga tatggcgaca attttgctat tagcaaagta gcccgggata tggagcagtt
    12601 gttggccacc aaaaatttgg agaagccact ggagcagccg gagaatgggt acacctacaa
    12661 ggccacctgg ttcatgcagt tccgggcggt cctgtggcga tcctggctgt cggtgctcaa
    12721 ggaaccactc ctcgtaaaag tgcgacttat tcagacaacg gtgagtggtt ccagtggaaa
    12781 caaatgatat aacgcttaca attcttggaa acaaattcgc tagattttag ttagaattgc
    12841 ctgattccac acccttctta gtttttttca atgagatgta tagtttatag ttttgcagaa
    12901 aataaataaa tttcatttaa ctcgcgaaca tgttgaagat atgaatatta atgagatgcg
    12961 agtaacattt taatttgcag atggttgcca tcttgattgg cctcatcttt ttgggccaac
    13021 aactcacgca agtgggcgtg atgaatatca acggagccat cttcctcttc ctgaccaaca
    13081 tgacctttca aaacgtcttt gccacgataa atgtaagtct tgtttagaat acatttgcat
    13141 attaataatt tactaacttt ctaatgaatc gattcgattt aggtgttcac ctcagagctg
    13201 ccagttttta tgagggaggc ccgaagtcga ctttatcgct gtgacacata ctttctgggc
    13261 aaaacgattg ccgaattacc gctttttctc acagtgccac tggtcttcac ggcgattgcc
    13321 tatccgatga tcggactgcg ggccggagtg ctgcacttct tcaactgcct ggcgctggtc
    13381 actctggtgg ccaatgtgtc aacgtccttc ggatatctaa tatcctgcgc cagctcctcg
    13441 acctcgatgg cgctgtctgt gggtccgccg gttatcatac cattcctgct ctttggcggc
    13501 ttcttcttga actcgggctc ggtgccagta tacctcaaat ggttgtcgta cctctcatgg
    13561 ttccgttacg ccaacgaggg tctgctgatt aaccaatggg cggacgtgga gccgggcgaa
    13621 attagctgca catcgtcgaa caccacgtgc cccagttcgg gcaaggtcat cctggagacg
    13681 cttaacttct ccgccgccga tctgccgctg gactacgtgg gtctggccat tctcatcgtg
    13741 agcttccggg tgctcgcata tctggctcta agacttcggg cccgacgcaa ggagtagccg
    13801 acatatatcc gaaataactg cttgtttttt tttttaccat tattaccatc gtgtttactg
    13861 tttattgccc cctcaaaaag ctaatgtaat tatatttgtg ccaataaaaa caagatatga
    13921 cctatagaat acaagtattt ccccttcgaa catccccaca agtagacttt ggatttgtct
    13981 tctaaccaaa agacttacac acctgcatac cttacatcaa aaactcgttt atcgctacat
    14041 aaaacaccgg gatatatttt ttatatacat acttttcaaa tcgcgcgccc tcttcataat
    14101 tcacctccac cacaccacgt ttcgtagttg ctctttcgct gtctcccacc cgctctccgc
    14161 aacacattca ccttttgttc gacgaccttg gagcgactgt cgttagttcc gcgcgattcg
    14221 gttcgctcaa atggttccga gtggttcatt tcgtctcaat agaaattagt aataaatatt
    14281 tgtatgtaca atttatttgc tccaatatat ttgtatatat ttccctcaca gctatattta
    14341 ttctaattta atattatgac tttttaaggt aattttttgt gacctgttcg gagtgattag
    14401 cgttacaatt tgaactgaaa gtgacatcca gtgtttgttc cttgtgtaga tgcatctcaa
    14461 aaaaatggtg ggcataatag tgttgtttat atatatcaaa aataacaact ataataataa
    14521 gaatacattt aatttagaaa atgcttggat ttcactggaa ctagaattaa ttcgg
//
