LOCUS       sequence-16658-e        1375 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 1375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L2682
FEATURES             Location/Qualifiers
     source          1..1375
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             555..575
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(665..687)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tgctgcggtc gactctagag gatcaccaaa aacggaacgt tgagctggac ggaaatagtg
       61 gtacagtgac atgattatag tttgaagatc tctaatttca caattagagc aaatgttgtt
      121 cggtatttat tttcaacggt atttatacta ttttccacct ttttctagaa cattcgagct
      181 gcttgttgca aaaggagggc gactcacatt cggtacatgg aaaagtagtg tacacaataa
      241 agagacccag atacattttc cgtctgcgtc tctttgcacc caccgggagt attttcaaac
      301 gaatgcatct aggaccttct agaacattct gtaaggctgc agaatgcggg tatataagga
      361 aagcgggctc agaggaagcc aacacgcttt gttctagtgc atctaaaaaa cttcgaaaat
      421 cctcatcgga tccccgggat tggccaaagg acccaaaggt atgtttcgaa tgatactaac
      481 ataacataga acattttcag gaggaccctt ggctagagga tccccgggta ccgagctcag
      541 aaaaaatgac tgctccaaag aagaagcgta aggtaccggt gggtgaagac cagaaacagc
      601 acctcgaact gagccgcgat attgcccagc gtttcaacgc gctgtatggc gagatcgatc
      661 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
      721 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
      781 cccaacagtt gcgaaggtaa gtttaaacag atccatacta actaacttgt tctgacataa
      841 ttttcagctt gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa gcggtgccgg
      901 aaagctggct ggagtgcgat cttcctgang ccgatactgt cgtcgtcccc tcnaactggc
      961 agatgcacgg ttacgatgcg cccatctacn ccaacgtgac ctatcccatn acggtcaatn
     1021 ccgccgtttg tncccncgga aanccnacng gntgttacnc nctcnntttn atggtgatga
     1081 aagctggcta cngnaagncn nacncnatta ttttganggg gtnactcggc gtttctcggg
     1141 gggcacgggg ctggtcgnta cgnccggant ttanttagtg accaactaca gacggattat
     1201 ttcggangcc ttgcncngan ttaccgaccn ttnccccgaa ancccccggg gggggccggn
     1261 gnggntttng naaaaatngg ggagggtttn nggcccttgg nancaanaan natcggnccc
     1321 ttngntnccn gnggggggcg gtaagnttna ngccntttng ggggggcnga ttttg
//
