LOCUS       sequence-166582-         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  csd2, construct: 121-308, pET21a(+) no-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   An DR, Im HN, Jang JY, Kim HS, Kim J, Yoon HJ, Hesek D, Lee M, 
            Mobashery S, Kim SJ, Suh SW
  TITLE     Structural Basis of the Heterodimer Formation between Cell 
            Shape-Determining Proteins Csd1 and Csd2 from Helicobacter pylori.
  JOURNAL   PLoS One. 2016 Oct 6;11(10):e0164243. doi: 
            10.1371/journal.pone.0164243. eCollection 2016.
  PUBMED    27711177
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-10-6"; volume: "11(10):e0164243. doi"; pages: " 
            10.1371/journal.pone.0164243. eCollection 2016"
COMMENT     Sequence Label: csd2_121-308/pET21a(+)
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             625..642
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(695..713)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      709..756
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(880..899)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttcccctcta gaaataattt tgtttaactt taagaaggag atatacatat gttagacaat
       61 ttgaatctgg ctcaaaaaca tttagcgctc atgctcattc ctaacggcat gcccataaaa
      121 acttatagcg ctatcaaacc caccaaagaa aggaaccacc ccattaaaaa aattaagggc
      181 gttgaatccg ggattgattt tatcgcgcca ttaaacacgc ctgtttatgc gagcgctgat
      241 gggattgtgg attttgtgaa gaccaattct aatgtggggt atgggaactt ggtgcgcatt
      301 gaacatgcgt ttggttttag ctccatttac acgcacttag atcatgtcaa tgtgcagccc
      361 aaaagcttca tccaaaaagg gcagttgatt ggctatagcg ggaagagcgg taatagcggc
      421 ggcgaaaaat tgcattatga agtgcgcttt ttgggtaaaa ttttagacgc gcaaaaattt
      481 ctggcatggg atttggatca ttttcaaagc gctttagaag aaaataaatt tattgaatgg
      541 aagaatttgt tttgggtttt agaagacatt gtccagctcc aagagcatgt ggataaagac
      601 gcactcataa gccagtaact cgagcaccac caccaccacc actgagatcc ggctgctaac
      661 aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc
      721 cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga
      781 ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
      841 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
      901 cctttctcgc cacgttc
//
